FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2325799013> ?p ?o ?g. }

• W2325799013 endingPage "87" @default.
• W2325799013 startingPage "77" @default.
• W2325799013 abstract "Background Drug resistance is a major limitation to the long-term efficacy of controlling chronic hepatitis B (CHB). There is a growing need to analyse multiple mutations associated with drug resistance because sequential or combinational use of antivirals is increasingly being used in treatment. In this study, we introduced a multiplex restriction fragment mass polymorphism (RFMP) assay for detecting mutations conferring entecavir and lamivudine resistance, and compared its performance with direct or clonal sequencing assays. Methods Multiplex PCR was performed with mixed primers designed to interrogate rt184, rt202, rt204 and rt250. The PCR products were digested with restriction enzymes and the resulting fragments were analysed by mass spectrometry. A total of 251 serum samples, taken serially from 45 patients who received entecavir treatment after confirmed diagnosis of lamivudine resistance and inadequate adefovir dipivoxil response, were analysed by the multiplex RFMP assay. Results The multiplex RFMP assay correctly identified known viral sequences with sufficient analytical sensitivity to detect as few as 100 IU/ml of HBV and with superior ability to determine haplotypes composed of neighbouring variations. Complex mutational patterns and relative abundances determined by multiplex RFMP assay were in good concordance with results obtained by direct or clonal sequencing analyses. Defined mixtures were successfully and consistently identified at 2% relative concentration of mutant versus wild-type virus by the assay. Conclusions The multiplex RFMP assay is an accurate and sensitive means to detect entecavir and lamivudine resistance mutations, simultaneously. The method is expected to enable early and efficient diagnosis of multiple drug resistance mutations for optimal management of CHB." @default.
• W2325799013 created "2016-06-24" @default.
• W2325799013 creator A5012207764 @default.
• W2325799013 creator A5033736236 @default.
• W2325799013 creator A5039586179 @default.
• W2325799013 creator A5059659062 @default.
• W2325799013 creator A5067263547 @default.
• W2325799013 creator A5083921432 @default.
• W2325799013 creator A5085080796 @default.
• W2325799013 creator A5087972459 @default.
• W2325799013 date "2011-01-01" @default.
• W2325799013 modified "2023-09-27" @default.
• W2325799013 title "Comparison of Multiplex Restriction Fragment Mass Polymorphism and Sequencing Analyses for Detecting Entecavir Resistance in Chronic Hepatitis B" @default.
• W2325799013 cites W1968393608 @default.
• W2325799013 cites W1984239612 @default.
• W2325799013 cites W2004100607 @default.
• W2325799013 cites W2031317669 @default.
• W2325799013 cites W2033608731 @default.
• W2325799013 cites W2044951291 @default.
• W2325799013 cites W2051090621 @default.
• W2325799013 cites W2051175845 @default.
• W2325799013 cites W2053738348 @default.
• W2325799013 cites W2065099361 @default.
• W2325799013 cites W2075498742 @default.
• W2325799013 cites W2080972647 @default.
• W2325799013 cites W2093604720 @default.
• W2325799013 cites W2101752294 @default.
• W2325799013 cites W2104389844 @default.
• W2325799013 cites W2110747022 @default.
• W2325799013 cites W2110847666 @default.
• W2325799013 cites W2126563189 @default.
• W2325799013 cites W2129352782 @default.
• W2325799013 cites W2132956128 @default.
• W2325799013 cites W2135386419 @default.
• W2325799013 cites W2137544368 @default.
• W2325799013 cites W2142557452 @default.
• W2325799013 cites W2144084705 @default.
• W2325799013 cites W2154915369 @default.
• W2325799013 cites W2155237439 @default.
• W2325799013 cites W2157564332 @default.
• W2325799013 cites W2338794667 @default.
• W2325799013 cites W2412556246 @default.
• W2325799013 cites W75419554 @default.
• W2325799013 doi "https://doi.org/10.3851/imp1702" @default.
• W2325799013 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21311111" @default.
• W2325799013 hasPublicationYear "2011" @default.
• W2325799013 type Work @default.
• W2325799013 sameAs 2325799013 @default.
• W2325799013 citedByCount "39" @default.
• W2325799013 countsByYear W23257990132012 @default.
• W2325799013 countsByYear W23257990132013 @default.
• W2325799013 countsByYear W23257990132014 @default.
• W2325799013 countsByYear W23257990132015 @default.
• W2325799013 countsByYear W23257990132016 @default.
• W2325799013 countsByYear W23257990132017 @default.
• W2325799013 countsByYear W23257990132019 @default.
• W2325799013 countsByYear W23257990132020 @default.
• W2325799013 countsByYear W23257990132021 @default.
• W2325799013 crossrefType "journal-article" @default.
• W2325799013 hasAuthorship W2325799013A5012207764 @default.
• W2325799013 hasAuthorship W2325799013A5033736236 @default.
• W2325799013 hasAuthorship W2325799013A5039586179 @default.
• W2325799013 hasAuthorship W2325799013A5059659062 @default.
• W2325799013 hasAuthorship W2325799013A5067263547 @default.
• W2325799013 hasAuthorship W2325799013A5083921432 @default.
• W2325799013 hasAuthorship W2325799013A5085080796 @default.
• W2325799013 hasAuthorship W2325799013A5087972459 @default.
• W2325799013 hasConcept C104317684 @default.
• W2325799013 hasConcept C114851261 @default.
• W2325799013 hasConcept C135763542 @default.
• W2325799013 hasConcept C153911025 @default.
• W2325799013 hasConcept C159047783 @default.
• W2325799013 hasConcept C168007829 @default.
• W2325799013 hasConcept C2522874641 @default.
• W2325799013 hasConcept C2776175608 @default.
• W2325799013 hasConcept C2777869810 @default.
• W2325799013 hasConcept C2779517838 @default.
• W2325799013 hasConcept C2780593183 @default.
• W2325799013 hasConcept C2781188995 @default.
• W2325799013 hasConcept C49105822 @default.
• W2325799013 hasConcept C54355233 @default.
• W2325799013 hasConcept C86803240 @default.
• W2325799013 hasConcept C90583042 @default.
• W2325799013 hasConceptScore W2325799013C104317684 @default.
• W2325799013 hasConceptScore W2325799013C114851261 @default.
• W2325799013 hasConceptScore W2325799013C135763542 @default.
• W2325799013 hasConceptScore W2325799013C153911025 @default.
• W2325799013 hasConceptScore W2325799013C159047783 @default.
• W2325799013 hasConceptScore W2325799013C168007829 @default.
• W2325799013 hasConceptScore W2325799013C2522874641 @default.
• W2325799013 hasConceptScore W2325799013C2776175608 @default.
• W2325799013 hasConceptScore W2325799013C2777869810 @default.
• W2325799013 hasConceptScore W2325799013C2779517838 @default.
• W2325799013 hasConceptScore W2325799013C2780593183 @default.
• W2325799013 hasConceptScore W2325799013C2781188995 @default.
• W2325799013 hasConceptScore W2325799013C49105822 @default.
• W2325799013 hasConceptScore W2325799013C54355233 @default.
• W2325799013 hasConceptScore W2325799013C86803240 @default.

• next