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- W2325850570 abstract "诱导表达GST-PR-1a蛋白,利用蛋白质内源荧光和外源ANS荧光为探针,对分离的重组表达蛋白进行热稳定性、盐酸胍、尿素研究.50~80℃处理时,PR-1a内源荧光和外源荧光几乎无变化,90℃时荧光强度均有明显降低.盐酸胍、尿素处理后,随着浓度的增加,最大吸收波长红移,说明PR-1a中色氨酸残基主要存在于分子内部,且易受温度等因素影响从而导致三维结构的变化.特定浓度的盐酸胍处理后,蛋白呈现中间态." @default.
- W2325850570 created "2016-06-24" @default.
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- W2325850570 creator A5029558354 @default.
- W2325850570 date "2015-01-01" @default.
- W2325850570 modified "2023-09-24" @default.
- W2325850570 title "温度、尿素、盐酸胍等因素对烟草蛋白PR-1a荧光光谱的影响研究" @default.
- W2325850570 hasPublicationYear "2015" @default.
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