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- W2326003969 abstract "To evaluate the biocompatibility of hydroxyapatite-coated titanium (HA/Ti) corneal implants at the molecular levels with histopathology.Eighty Sprague-Dawley rats were divided into 2 equal groups. In the study group, HA/Ti prosthetics were implanted into the right corneal stroma. The control group received a sham incision. Corneas were collected and studied with histopathological examination (HE), immunohistochemical, and other stains, including scanned electron microscopy and reverse transcription-polymerase chain reaction, to evaluate inflammatory reactions, tissue repair, and expression of various biological factors during healing.In the control group, corneal neovascularization occurred 7 days after surgery, and the corneas recovered 28 days after surgery. In the study group, corneal vascularization increased substantially on day 7 and stabilized on day 28. For both groups, reverse transcription-polymerase chain reaction detected expressions of 6 primers at all time points. The amplified sequences were consistent with the designed sequences. The expression of matrix metalloproteinase-2, matrix metalloproteinase-9, bFGF, vascular endothelial growth factor, and type III collagen were delayed in the study group compared with the control group. Histological analyses showed a tight attachment of the corneal tissue to the HA/Ti implant on day 28.The HA/Ti corneal implants can remain stable in corneal tissue for a long time, induce corneal neovascularization, and stimulate inflammatory cells and keratocytes to synthesize or activate matrix metalloproteinases. Artificial cornea made from this material show enhanced stability and biocompatibility in vivo." @default.
- W2326003969 created "2016-06-24" @default.
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- W2326003969 date "2011-01-01" @default.
- W2326003969 modified "2023-09-23" @default.
- W2326003969 title "Implantation of Hydroxyapatite–Titanium Corneal Implants in Rat Cornea" @default.
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- W2326003969 doi "https://doi.org/10.1097/ico.0b013e3181d92817" @default.
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