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- W2326007095 abstract "Previous studies have shown that nm23-H1 is a tumor metastasis suppressor gene. Nucleotide diphosphate kinase 1 (NDPK1) encoded by nm23-H1 is involved in cancer cellular differentiation, proliferation, apoptosis and metastasis. This study was to investigate the effects of nm23-H1 on proliferation and invasion of cervical cancer cells.The eukaryotic expression vector pcDNA3.1-nm23-H1 was transfected into cervical cancer cells. Cell invasion potential was determined by the Transwell assay. Cell proliferation was measured by MTT assay and changes in cell cycle distribution were analyzed by flow cytometry (FCM).Compared with parent cells (Caski and SiHa) and vector control cells (Caski-3.1 and SiHa-3.1), the proliferation and invasion of pcDNA3.1-nm23-H1 transfected cells (SiHa-N and Caski-N) were apparently decreased (P<0.05); the proportions of G2/M and S cells were obviously decreased while that of G0/G1 cells was increased (P<0.05). However, transfection of nm23-H1 gene had no influence on proliferation, cell cycle and invasion of HeLa cells (P>0.05).nm23-H1 gene could inhibit proliferation and invasion of cervical cancer cells in a cell-dependent manner." @default.
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- W2326007095 date "2009-07-05" @default.
- W2326007095 modified "2023-09-27" @default.
- W2326007095 title "Effects of tumor metastasis suppressor gene nm23-H1 on invasion and proliferation of cervical cancer cell lines" @default.
- W2326007095 doi "https://doi.org/10.5732/cjc.008.10719" @default.
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