Matches in SemOpenAlex for { <https://semopenalex.org/work/W2326251330> ?p ?o ?g. }
- W2326251330 endingPage "920" @default.
- W2326251330 startingPage "906" @default.
- W2326251330 abstract "The viral infectivity factor (Vif) is essential for the productive infection and dissemination of HIV-1 in non-permissive cells, containing the cellular anti-HIV defense cytosine deaminases APOBEC3 (A3G and A3F). Vif neutralizes the antiviral activities of the APOBEC3G/F by diverse mechanisms including their degradation through the ubiquitin/proteasome pathway and their translational inhibition. In addition, Vif appears to be an active partner of the late steps of viral replication by interacting with Pr55(Gag), reverse transcriptase and genomic RNA. Here, we expressed and purified full-length and truncated Vif proteins, and analyzed their RNA binding and chaperone properties. First, we showed by CD and NMR spectroscopies that the N-terminal domain of Vif is highly structured in solution, whereas the C-terminal domain remains mainly unfolded. Both domains exhibited substantial RNA binding capacities with dissociation constants in the nanomolar range, whereas the basic unfolded C-terminal domain of Vif was responsible in part for its RNA chaperone activity. Second, we showed by NMR chemical shift mapping that Vif and NCp7 share the same binding sites on tRNA(Lys) 3, the primer of HIV-1 reverse transcriptase. Finally, our results indicate that Vif has potent RNA chaperone activity and provide direct evidence for an important role of the unstructured C-terminal domain of Vif in this capacity." @default.
- W2326251330 created "2016-06-24" @default.
- W2326251330 creator A5006554668 @default.
- W2326251330 creator A5043443505 @default.
- W2326251330 creator A5046340967 @default.
- W2326251330 creator A5059235424 @default.
- W2326251330 creator A5079724200 @default.
- W2326251330 creator A5083671890 @default.
- W2326251330 creator A5089354223 @default.
- W2326251330 date "2014-07-01" @default.
- W2326251330 modified "2023-09-24" @default.
- W2326251330 title "Characterization of RNA binding and chaperoning activities of HIV-1 Vif protein" @default.
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- W2326251330 doi "https://doi.org/10.4161/rna.29546" @default.
- W2326251330 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4179964" @default.