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• W2326329179 endingPage "8581" @default.
• W2326329179 startingPage "8575" @default.
• W2326329179 abstract "Matrix-assisted laser desorption/ionization (MALDI) based mass spectrometric imaging (MSI) is increasingly being used as an analytical tool to evaluate the molecular makeup of tissue samples. From the direct analysis of a tissue section, the physical integrity of sample is preserved; thus, spatial information of a compound's distribution may be determined. One limitation of the technique, however, has been the inability to determine the absolute concentration from a tissue sample. Here we report the development of a quantitative MSI technique in which the distribution of acetyl-L-carnitine (AC) in a piglet brain sample is quantified with MALDI MSI. An isotopically labeled internal standard was applied uniformly beneath the tissue section, and wide-isolation tandem mass spectrometry was performed. Normalizing the analyte ion signal by the internal standard ion signal resulted in significant improvements in MS images, signal reproducibility, and calibration curve linearity. From the improved MS images, the concentration of AC was determined and plotted producing a concentration-scaled image of the distribution of AC in the piglet brain section." @default.
• W2326329179 created "2016-06-24" @default.
• W2326329179 creator A5031735945 @default.
• W2326329179 creator A5048042532 @default.
• W2326329179 date "2011-10-17" @default.
• W2326329179 modified "2023-10-14" @default.
• W2326329179 title "Quantitative Tandem Mass Spectrometric Imaging of Endogenous Acetyl-<scp>l</scp>-carnitine from Piglet Brain Tissue Using an Internal Standard" @default.
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• W2326329179 doi "https://doi.org/10.1021/ac201949b" @default.
• W2326329179 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21942933" @default.
• W2326329179 hasPublicationYear "2011" @default.
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