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• W2326491569 abstract "Fluorescence polarization measurement was applied to detect the impaired Con A-induced mitogenic responses in tumor-bearing mice. Spleen lymphocytes from normal C3H/HeN mice and from those inoculated 1×106 Ehrlich ascites tumor cells were treated with Con A and examined for their fluorescence polarization changes (P-value) using fluorescein diacetate and a polarization spectrofluorometer. Murine spleen cells when stimulated by 5μg/ml of Con A at 37°C for 45 min showed a high polarization change with a reduction rate of p-value, 0.79±0.03 (n=33). This Con A-induced polarization change of spleen cells was almost completely inhibited by α-methylmannoside, a competitive inhibitor of Con A-binding to the cell surface. Spleen lymphocytes from tumor-bearing mice showed much less polarization changes with a reduction rate of P-value, 0.91±0.04 (n=33). This low fluorescence polarization change appeared as early as 3 days after tumor inoculation. Impairement of mitogenic responses as revealed by 3H-thymidine uptake in tumor-bearing mice also appeared 3 days after tumor inoculation. These results indicate that the fluorescence polarization measurement may be a valuable tools for the detection of impaired mitogenic responses of lymphocytes in tumor-bearing mice." @default.
• W2326491569 created "2016-06-24" @default.
• W2326491569 creator A5070234945 @default.
• W2326491569 date "1980-01-01" @default.
• W2326491569 modified "2023-09-25" @default.
• W2326491569 title "Fluorescence polarization assay of mitogenic response of lymphocytes" @default.
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• W2326491569 doi "https://doi.org/10.2330/joralbiosci1965.22.614" @default.
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