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• W2326571885 abstract "Event Abstract Back to Event Gel based proteomics without a genome: an EST based approach in Schistocerca gregaria Bart Boerjan1*, Koen Vandingenen1, Arnold De Loof1 and Liliane Schoofs1 1 Catholic University of Leuven, Department of Biology, Belgium The enigmatic phenomenon of phase polyphenism in the desert locust (Schistocerca gregaria) has been studied in many ways. Both behavioural and morphological studies have been performed over the years. Each yielded specific insights in this biological mechanism that has enormous consequences from a humanitarian as well as an economical point of view. The lack of genomic information in Schistocerca hampers the application of rapidly evolving state of the art technology to locust studies. For example, differential proteomics could be used to analyze differences at the proteome level in both the solitary and gregarious phase, hereby revealing the players that are taking part in phase polyphenism and transition. Without genomic information however, it is difficult to analyse proteomics data in a straightforward manner by means of mass spectrometric approaches. This study provides an approach to identify protein spots from gel based proteomics studies on the prothoracic glands of the desert locust. The prothoracic gland takes part in ecdysteroid production which is necessary for insects to molt. However, there are clear differences in the morphological structure and presence of the glands in both phases, which make this an interesting tissue to study in the frame of phase polyphenism. We combine the classical identification workflow of tryptic digests with a bioinformatics software package (Peaks studio, Bioinformatics Solutions inc.). Together with partial genomic information in the format of an Schistocerca EST-library and the DE NOVO sequencing of tryptic peptides we have been able to identify a significant part of visualised proteins and hereby provide a first step to implement modern proteomics technologies in locust research. Keywords: comparative endocrinology Conference: 25th Conference of the European Comparative Endocrinologists, Pécs, Hungary, 31 Aug - 4 Sep, 2010. Presentation Type: Conference Presentation Topic: Comparative endocrinology Citation: Boerjan B, Vandingenen K, De Loof A and Schoofs L (2010). Gel based proteomics without a genome: an EST based approach in Schistocerca gregaria. Front. Endocrinol. Conference Abstract: 25th Conference of the European Comparative Endocrinologists. doi: 10.3389/conf.fendo.2010.01.00015 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 26 Aug 2010; Published Online: 29 Aug 2010. * Correspondence: Dr. Bart Boerjan, Catholic University of Leuven, Department of Biology, Leuven, Belgium, bart.boerjan@bio.kuleuven.be Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Bart Boerjan Koen Vandingenen Arnold De Loof Liliane Schoofs Google Bart Boerjan Koen Vandingenen Arnold De Loof Liliane Schoofs Google Scholar Bart Boerjan Koen Vandingenen Arnold De Loof Liliane Schoofs PubMed Bart Boerjan Koen Vandingenen Arnold De Loof Liliane Schoofs Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page." @default.
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• W2326571885 date "2010-01-01" @default.
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• W2326571885 title "Gel based proteomics without a genome: an EST based approach in Schistocerca gregaria" @default.
• W2326571885 doi "https://doi.org/10.3389/conf.fendo.2010.01.00015" @default.
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