Matches in SemOpenAlex for { <https://semopenalex.org/work/W2326977634> ?p ?o ?g. }
- W2326977634 endingPage "767" @default.
- W2326977634 startingPage "759" @default.
- W2326977634 abstract "In order to investigate whether the positive effect of adrenergic stimulation on lipoprotein lipase (LPL) gene expression in brown adipose tissue is a direct effect on the brown adipocytes themselves, the expression of the LPL gene was investigated by measuring LPL mRNA levels in brown adipocytes, isolated as precursors from the brown adipose tissue of rats and grown in culture in a fully defined medium before experimentation. Addition of noradrenaline led to an enhancement of LPL gene expression; the mRNA levels increased as a linear function of time for at least 5 h and were finally approx. 3 times higher than in control cells, an increase commensurate with that seen in vivoin both LPL mRNA levels and LPL activity during physiological stimulation. The increase was dependent on transcription. The effect of noradrenaline showed simple MichaelisŐMenten kinetics with an EC50 of approx. 11 nM. β3-Agonists (BRL-37344 and CGP-12177) could mimic the effect of noradrenaline; the β1-agonist dobutamine and the β2-agonist salbutamol could not; the α1-agonist cirazoline had only a weak effect. The effect of noradrenaline was fully inhibited by the β-antagonist propranolol and was halved by the α1-antagonist prazosin; the α2-antagonist yohimbine was without effect. An increase in LPL mRNA level similar to (but not significantly exceeding) that caused by noradrenaline could also be induced by the cAMP-elevating agents forskolin and cholera toxin, and 8-Br-cAMP also increased LPL mRNA levels. The increase in LPL gene expression was not mediated via an increase in the level of an intermediary proteinaceous factor. It is concluded that the physiologically induced increase in LPL gene expression is a direct effect of noradrenaline on the brown adipocytes themselves, mediated via a dominant β3-adrenergic pathway and an auxillary α1-adrenergic pathway which converge at a regulatory point in transcriptional control." @default.
- W2326977634 created "2016-06-24" @default.
- W2326977634 creator A5013959249 @default.
- W2326977634 creator A5015266255 @default.
- W2326977634 creator A5031816335 @default.
- W2326977634 creator A5045702595 @default.
- W2326977634 creator A5089321330 @default.
- W2326977634 date "1997-02-01" @default.
- W2326977634 modified "2023-10-18" @default.
- W2326977634 title "Adrenergic stimulation of lipoprotein lipase gene expression in rat brown adipocytes differentiated in culture: mediation via β3- and α1-adrenergic receptors" @default.
- W2326977634 cites W104868368 @default.
- W2326977634 cites W1485810221 @default.
- W2326977634 cites W1488742914 @default.
- W2326977634 cites W1501975414 @default.
- W2326977634 cites W1509358926 @default.
- W2326977634 cites W1513078811 @default.
- W2326977634 cites W1529485901 @default.
- W2326977634 cites W1550576135 @default.
- W2326977634 cites W1583120969 @default.
- W2326977634 cites W1594004211 @default.
- W2326977634 cites W1604930697 @default.
- W2326977634 cites W1681732703 @default.
- W2326977634 cites W1772836693 @default.
- W2326977634 cites W1785407489 @default.
- W2326977634 cites W1791831929 @default.
- W2326977634 cites W1917787915 @default.
- W2326977634 cites W1947850437 @default.
- W2326977634 cites W1966575057 @default.
- W2326977634 cites W1967296335 @default.
- W2326977634 cites W1968513394 @default.
- W2326977634 cites W1969222263 @default.
- W2326977634 cites W1969299547 @default.
- W2326977634 cites W1970083668 @default.
- W2326977634 cites W1983015221 @default.
- W2326977634 cites W1995178988 @default.
- W2326977634 cites W2004304269 @default.
- W2326977634 cites W2008190970 @default.
- W2326977634 cites W2010751700 @default.
- W2326977634 cites W2012113206 @default.
- W2326977634 cites W2019374918 @default.
- W2326977634 cites W2028671417 @default.
- W2326977634 cites W2042856235 @default.
- W2326977634 cites W2044816854 @default.
- W2326977634 cites W2050661928 @default.
- W2326977634 cites W2051599797 @default.
- W2326977634 cites W2051908897 @default.
- W2326977634 cites W2060324183 @default.
- W2326977634 cites W2061148091 @default.
- W2326977634 cites W2061776195 @default.
- W2326977634 cites W2065705524 @default.
- W2326977634 cites W2070293541 @default.
- W2326977634 cites W2075027229 @default.
- W2326977634 cites W2075807637 @default.
- W2326977634 cites W2090278673 @default.
- W2326977634 cites W2094251732 @default.
- W2326977634 cites W2117854673 @default.
- W2326977634 cites W2122087475 @default.
- W2326977634 cites W2129138669 @default.
- W2326977634 cites W2137347569 @default.
- W2326977634 cites W2180134313 @default.
- W2326977634 cites W2182826466 @default.
- W2326977634 cites W2184918479 @default.
- W2326977634 cites W2238343100 @default.
- W2326977634 cites W2305728397 @default.
- W2326977634 cites W2340779636 @default.
- W2326977634 cites W2405011955 @default.
- W2326977634 cites W2410090091 @default.
- W2326977634 cites W2410973294 @default.
- W2326977634 cites W2413190348 @default.
- W2326977634 cites W2414168004 @default.
- W2326977634 cites W2415390087 @default.
- W2326977634 cites W2415469316 @default.
- W2326977634 cites W2438952504 @default.
- W2326977634 cites W2467770782 @default.
- W2326977634 cites W349293835 @default.
- W2326977634 cites W39315710 @default.
- W2326977634 cites W67917582 @default.
- W2326977634 doi "https://doi.org/10.1042/bj3210759" @default.
- W2326977634 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1218133" @default.
- W2326977634 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9032464" @default.
- W2326977634 hasPublicationYear "1997" @default.
- W2326977634 type Work @default.
- W2326977634 sameAs 2326977634 @default.
- W2326977634 citedByCount "24" @default.
- W2326977634 countsByYear W23269776342014 @default.
- W2326977634 countsByYear W23269776342016 @default.
- W2326977634 countsByYear W23269776342017 @default.
- W2326977634 countsByYear W23269776342019 @default.
- W2326977634 countsByYear W23269776342020 @default.
- W2326977634 crossrefType "journal-article" @default.
- W2326977634 hasAuthorship W2326977634A5013959249 @default.
- W2326977634 hasAuthorship W2326977634A5015266255 @default.
- W2326977634 hasAuthorship W2326977634A5031816335 @default.
- W2326977634 hasAuthorship W2326977634A5045702595 @default.
- W2326977634 hasAuthorship W2326977634A5089321330 @default.
- W2326977634 hasBestOaLocation W23269776342 @default.
- W2326977634 hasConcept C104317684 @default.
- W2326977634 hasConcept C126322002 @default.