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- W2327146571 abstract "Currently, the chromatin immunoprecipitation assay (ChIP) is the most powerful method utilized to selectively enrich for DNA sequences bound by a particular protein in living cells. We developed a SOLiD™ ChIP-seq kit, which offers an optimized ChIP workflow and an efficient SOLiD™ ChIP-seq library construction from relatively low cell number samples. ChIP procedure is usually laborious, time consuming, and typically requires large starting cell numbers. We use MAGnify™ Chromatin Immunoprecipitation System, which is suitable for fast enrichment of chromatin complexes and DNA recovery. It is able to use lower starting cell numbers for ChIP and allows faster throughput to investigate different chromatin and transcription time-course events as well as enable antibody screening to determine ChIP compatibility. In addition, we develop a sensitive ChIP-seq library construction procedure which enables users to construct a complex library using as low as 1 ng ChIP DNA. Combining with SOLiD™9s ultra high sequencing throughput, SOLiD™ ChIP-seq offers a highly sensitive, hypothesis-neutral approach to accurately characterize protein-DNA interactions at genome-wide scale. Here we paired the SOLiD™ Fragment Library Barcoding Kit, which enables users to pool fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, with the SOLiD™ ChIP-Seq Kit to characterize Estrogen Receptor alpha binding sites in breast cancer cells utilizing the SOLiD™ platform. In addition, we demonstrate a novel, rapid, and low input per ChIP tissue collection protocol for researchers utilizing solid mammalian tissue to study protein-DNA interaction via the MAGnify™ ChIP System. The 50 cent assay is reduced to less than 1 cent per reaction and it reduces reported homebrew amount of tissue from approximately 30mg to less than 1mg per ChIP reaction when paired with the powerful MAGnify™ ChIP system. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 3002. doi:10.1158/1538-7445.AM2011-3002" @default.
- W2327146571 created "2016-06-24" @default.
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- W2327146571 date "2011-04-15" @default.
- W2327146571 modified "2023-09-27" @default.
- W2327146571 title "Abstract 3002: Multiplexing ChIP-Seq and rapid chromatin preparation from solid mammalian tissues for low cell ChIP assays" @default.
- W2327146571 doi "https://doi.org/10.1158/1538-7445.am2011-3002" @default.
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