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- W2327166277 abstract "Tissue distribution of epinermal growth-factor (EGF) in rat was investigated using a human EGF (hEGF) radioimmunossay system. Antisera generated against hEGF reacted with hEGF and [21 Leu] hEGF but not with mouse EGF, fibroblast growth factor (FGF), nerve growth factor (NGF), platelet derived growth factor (PDGF), and endothelial cell growth supplement (ECGS). Similar amounts of EGF immunoreactivity (EGF-IR) were found in the rat submandibular gland (32.8±4.59ng/g tissue, n= 5) and duodenum (39.9± 18.7ng/g tissue, n= 9), whereas little EGF was detected in other tissues. No sex difference was observed in submandibular and duodenal EGF-IR, being different from the case of mouse in which ten times higher concentration of EGF-IR was found in male submandibular gland. Sephadex G-50 gel chromatography of submandibular and duodenal extracts revealed that each consisted of at least three different immunoreactive forms. The predominant EGF-IR was coeluted with 125 I-EGF, significant amounts of EGF-IR was observed in the void volume fractions, and only small peaks of EGF-IR were present near Vt. These data clarified the presence of EGF-IR in rat submandibular and duodenal tissues and the molecular heterogeneity of EGF-IR and suggest some important role of EGF in the gastrointestinal function." @default.
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- W2327166277 date "1988-01-01" @default.
- W2327166277 modified "2023-09-23" @default.
- W2327166277 title "Study on Distribution of Epidermal Growth Factor (EGF) in Rat Tissues" @default.
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- W2327166277 doi "https://doi.org/10.1507/endocrine1927.64.7_593" @default.
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