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• W2327447915 abstract "OZET Objectives: Hepatitis B virus (HBV) infection is a major global public health problem. Determination of serum markers is crucial for rapid screening and clinical diagnosis of HBV infection. The detection of hepatitis B surface antijen (HBsAg) demands highly sensitive and specific immunoassays. The objective of this study was to compare technical performance of the Chemiluminescence Microparticle Immunoassay (CMIA) and Electrochemiluminescence Immunoassay (ECLIA) for detection of HBsAg. Materials and Methods: The total number of serum samples tested was 197 by using two different automated immunoassays (Modular E170 assay and Architect i1000). Sixty-six of the samples were stored HBsAg reactive samples from blood donors that were tested and stored previously by Microelisa (Triturus-Microelisa analyser) method and 131 of them were routine clinical samples. If there were any discrepant results between two methods, serum samples also tested for anti-HBctotal and HBV-DNA (Cobas Tagman 48 Roche) for confirming test results. Results: The sensitivity of HBsAg tests was found to be 100% and 98% for ECLIA and CMIA methods, respectively. The specificity of HBsAg tests was found to be 99% and 97% for ECLIA and CMIA methods, respectively. The result of correlation analysis between the two methods was 75%. Conclusion: In this study, ECLIA and CMIA methods were compared for the detection of HBsAg from blood donor samples and routine clinical samples. There was a significant correlation between the assay results of the two methods. Both methods were highly compatible with each other and they were found to be suitable and reliable for routine HBsAg screening. (Viral Hepatitis Journal 2014; 20(3): 101-105)" @default.
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• W2327447915 date "2014-12-05" @default.
• W2327447915 modified "2023-10-18" @default.
• W2327447915 title "Comparison of Chemiluminescence Microparticle Immunoassay and Electrochemiluminescence Immunoassay for Detection of HBsAg" @default.
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• W2327447915 doi "https://doi.org/10.4274/vhd.08760" @default.
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