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• W2327565516 abstract "The supported lipid bilayer (SLB) is a well-known system for studying the cell membrane and membrane proteins. It is also promising as a platform for studying cell processes: the cell adhesion, the cell membrane receptors, and the intercellular signaling processes. SLBs made of natural lipids appeared to be protein and cell repellent. Thus, to use the SLB as a substrate for cells, one should functionalize them to provide adhesion. In the present paper, we describe a simple approach to promote adhesion of neuronal cells to the SLB without using proteins or peptides, by introducing positively charged lipids 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) into the SLB made of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). We show that neurons adhere to these bilayers and grow for at least 10 days. The SLBs themselves were found to degrade with time in cell culture conditions, but maintained fluidity (as revealed by fluorescence recovery after photobleaching), demonstrating the possibility of using SLBs for studying neuronal cells in culture." @default.
• W2327565516 created "2016-06-24" @default.
• W2327565516 creator A5030253617 @default.
• W2327565516 creator A5045587732 @default.
• W2327565516 date "2012-09-06" @default.
• W2327565516 modified "2023-10-14" @default.
• W2327565516 title "Positively Charged Supported Lipid Bilayers as a Biomimetic Platform for Neuronal Cell Culture" @default.
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• W2327565516 doi "https://doi.org/10.1021/la302500r" @default.
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