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• W2327566944 endingPage "1936" @default.
• W2327566944 startingPage "1923" @default.
• W2327566944 abstract "The present study showed that human demineralized dentin matrix gelatin (DNIG) induced prolific hyaline cartilage in rat foetal muscle tissue in vitro, and that bone morphogenetic protein (BMP) extracted from human DMG induced bone in vivo when it was implanted subcutaneously in a rat. Extracellular matrix of the cartilage-producing tissue was histologically and histochemically investigated in tissue culture of rat foetal muscle tissue on human DMG. At the prechondrogenic stage, many fibroblastic cells developed in the area neighboring DMG and secreted extracellular matrix containing much hyaluronic acid. Then, alkaline phosphatase (ALP) activity of plasma membrane of the fibroblastic cells became higher. The extracellular matrix decreased in proportion to increase of ALP activity, and the intercellular space became smaller. Later, the fibroblastic cells secreted extracellular matrix containing much sulphated glycosaminoglycans, and the intercellular space spread again. These cells changed into ovoid chondrocytes in the area next to DMG. The chondrocytes secreted type II collagen in the extracellular space and ALP activity disappeared. Fibroblastic cells which proliferated in the surrounding area of cartilage exhibited ALP activity. ALP activity in this course is one of good indicators for predicting cartilage formation. It can be assumed that ALP activity correlates with qualitative alteration of extracellular matrix in an early stage of chondrogenesis." @default.
• W2327566944 created "2016-06-24" @default.
• W2327566944 creator A5090190318 @default.
• W2327566944 date "1991-01-01" @default.
• W2327566944 modified "2023-09-24" @default.
• W2327566944 title "Experimental studies on induction of cartilage and bone by human dentin matrix." @default.
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• W2327566944 doi "https://doi.org/10.5794/jjoms.37.1923" @default.
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