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- W2327607690 abstract "A membrane fraction was isolated by differential centrifugation from PVX-inoculated tobacco protoplasts to investigate in vitro viral RNA synthesis. This fraction was capable of catalyzing the in vitro synthesis of a genomic-length (6.4kb) and two subgenomic-length RNAs (2.1 and 0.9kb). The different sensitivities of the RNAs to S1 nuclease at high and low salt concentrations suggested that the synthesized genomic-length RNA was mainly the replicative intermediate RNA (RI) and that the two subgenomic-length RNAs were single-stranded. Most of the synthesized RNAs were shown to be of a positive polarity based on ribonuclease protection assay. Dependent on the exogenous template, the fraction treated with micrococcal nuclease synthesized only the genomic-length RNA but not the subgenomic RNAs. Treating the membrane fraction with protease abolished synthesis of the subgenomic-length RNAs, but not that of genomic-length RNA. The synthesizing activity for the subgenomiclength RNAs was released into the soluble fraction after treatment with the detergent Brij 58. These results suggested that genomic and subgenomic RNAs are replicated at different sites in the membrane; genomic RNA synthesis occurs inside the membranes, whereas subgenomic RNA synthesis occurs on their surface." @default.
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- W2327607690 date "1998-01-01" @default.
- W2327607690 modified "2023-10-04" @default.
- W2327607690 title "In Vitro Viral RNA Synthesis in a Crude Membrane Fraction from Tobacco Protoplasts Inoculated with Potato Virus X." @default.
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- W2327607690 doi "https://doi.org/10.3186/jjphytopath.64.85" @default.
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