Matches in SemOpenAlex for { <https://semopenalex.org/work/W2327785514> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2327785514 abstract "Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FLEpigallocatechin-3-gallate (EGCG) has been reported to affect many cellular regulatory pathways. This study aims to determine whether EGCG could target microRNA, one of the mechanisms for cells to achieve subtle change in multiple targets. We found that, in both human and mouse lung cancer cells in culture, EGCG specifically upregulated the expression of mir-210, a major microRNA regulated by HIF1α. Furthermore, we found that overexpression of mir-210 led to reduced cell proliferation rate and anchorage-independent growth as well as reduced sensitivity to EGCG. To explore the mechanisms of mir-210 regulation by EGCG, we demonstrated that the regulation was mediated through hypoxia-response element in mir-210 promoter. Consistently, the upregulation of mir-210 was found to be correlated with the transiently stabilized HIF1α in lung cancer cell lines after EGCG treatment. Such stabilization was further shown by the stabilization of HA-tagged HIF1α, but not the P402A/P564A mutated HIF1α, by EGCG, suggesting that EGCG targets the oxygen-dependent degradation (ODD) domain. Direct evidence was obtained by affinity binding assay showing that EGCG specifically binds HIF1α with a Kd=3.47 μM. This result implies that EGCG binding interferes with the hydroxylation of key Pro residues in the ODD domain, preventing HIF1α from the Pro hydroxylation-induced ubiquitination and subsequent proteosome-mediated degradation. In summary, our results demonstrate the elevation of mir-210 by EGCG and this is mediated by the stabilization of HIF1α. This event may contribute to the anti-cancer activity of EGCG (Supported by NIH grant CA 122474 and CA133021).Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 1870. doi:10.1158/1538-7445.AM2011-1870" @default.
- W2327785514 created "2016-06-24" @default.
- W2327785514 creator A5033020983 @default.
- W2327785514 creator A5082286933 @default.
- W2327785514 creator A5086188593 @default.
- W2327785514 date "2011-04-15" @default.
- W2327785514 modified "2023-09-27" @default.
- W2327785514 title "Abstract 1870: Green tea polyphenol EGCG upregulates mir-210 expression in lung cancer cells by transiently stabilizing HIF1α" @default.
- W2327785514 doi "https://doi.org/10.1158/1538-7445.am2011-1870" @default.
- W2327785514 hasPublicationYear "2011" @default.
- W2327785514 type Work @default.
- W2327785514 sameAs 2327785514 @default.
- W2327785514 citedByCount "0" @default.
- W2327785514 crossrefType "proceedings-article" @default.
- W2327785514 hasAuthorship W2327785514A5033020983 @default.
- W2327785514 hasAuthorship W2327785514A5082286933 @default.
- W2327785514 hasAuthorship W2327785514A5086188593 @default.
- W2327785514 hasConcept C104317684 @default.
- W2327785514 hasConcept C121608353 @default.
- W2327785514 hasConcept C127561419 @default.
- W2327785514 hasConcept C145059251 @default.
- W2327785514 hasConcept C181199279 @default.
- W2327785514 hasConcept C185592680 @default.
- W2327785514 hasConcept C2781109383 @default.
- W2327785514 hasConcept C502942594 @default.
- W2327785514 hasConcept C54355233 @default.
- W2327785514 hasConcept C55493867 @default.
- W2327785514 hasConcept C62112901 @default.
- W2327785514 hasConcept C81885089 @default.
- W2327785514 hasConcept C86803240 @default.
- W2327785514 hasConcept C95444343 @default.
- W2327785514 hasConcept C96232424 @default.
- W2327785514 hasConceptScore W2327785514C104317684 @default.
- W2327785514 hasConceptScore W2327785514C121608353 @default.
- W2327785514 hasConceptScore W2327785514C127561419 @default.
- W2327785514 hasConceptScore W2327785514C145059251 @default.
- W2327785514 hasConceptScore W2327785514C181199279 @default.
- W2327785514 hasConceptScore W2327785514C185592680 @default.
- W2327785514 hasConceptScore W2327785514C2781109383 @default.
- W2327785514 hasConceptScore W2327785514C502942594 @default.
- W2327785514 hasConceptScore W2327785514C54355233 @default.
- W2327785514 hasConceptScore W2327785514C55493867 @default.
- W2327785514 hasConceptScore W2327785514C62112901 @default.
- W2327785514 hasConceptScore W2327785514C81885089 @default.
- W2327785514 hasConceptScore W2327785514C86803240 @default.
- W2327785514 hasConceptScore W2327785514C95444343 @default.
- W2327785514 hasConceptScore W2327785514C96232424 @default.
- W2327785514 hasLocation W23277855141 @default.
- W2327785514 hasOpenAccess W2327785514 @default.
- W2327785514 hasPrimaryLocation W23277855141 @default.
- W2327785514 hasRelatedWork W1993473709 @default.
- W2327785514 hasRelatedWork W2000947590 @default.
- W2327785514 hasRelatedWork W2102482931 @default.
- W2327785514 hasRelatedWork W2249199135 @default.
- W2327785514 hasRelatedWork W2315901472 @default.
- W2327785514 hasRelatedWork W2316639253 @default.
- W2327785514 hasRelatedWork W2317221914 @default.
- W2327785514 hasRelatedWork W2327180642 @default.
- W2327785514 hasRelatedWork W2333051949 @default.
- W2327785514 hasRelatedWork W2489832387 @default.
- W2327785514 hasRelatedWork W2592717980 @default.
- W2327785514 hasRelatedWork W2620613525 @default.
- W2327785514 hasRelatedWork W2783754843 @default.
- W2327785514 hasRelatedWork W2789998961 @default.
- W2327785514 hasRelatedWork W2886767753 @default.
- W2327785514 hasRelatedWork W2908943926 @default.
- W2327785514 hasRelatedWork W3019408297 @default.
- W2327785514 hasRelatedWork W3022861793 @default.
- W2327785514 hasRelatedWork W3118767005 @default.
- W2327785514 hasRelatedWork W3184322793 @default.
- W2327785514 isParatext "false" @default.
- W2327785514 isRetracted "false" @default.
- W2327785514 magId "2327785514" @default.
- W2327785514 workType "article" @default.