Matches in SemOpenAlex for { <https://semopenalex.org/work/W2328000568> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2328000568 endingPage "e237" @default.
- W2328000568 startingPage "e236" @default.
- W2328000568 abstract "We report a versatile approach for the colorimetric assay of alkaline phosphatase (ALP) activity based on the distinctive metal-to-ligand charge-transfer (MLCT) absorption properties of Fe(II)-phenanthroline reporter. In the presence of ALP, the applied substrate ascorbic acid 2-phosphate is enzymatically hydrolyzed to produce ascorbic acid, which then reduces Fe3+ to Fe2+. The complexation of Fe2+ with the bathophenanthroline disulfonate (BPS) ligand generates a blood-red Fe(BPS)34− reporter, which is characterized by an intense MLCT absorption band at 535 nm in the visible range. Under optimal conditions, the spectral output exhibits a good quantitative relationship with ALP activity over the range of 0–220 mU mL−1 with a detection limit of 0.94 mU mL−1. Moreover, the activity of ALP can also be conveniently judged through naked-eye observations. Results indicate that it is highly selective and can be applied to the screening of ALP inhibitors. In addition, it has been successfully employed to detect the endogenous ALP level of undiluted human serum samples, with a detection limit of 1.05 mU mL−1 being achieved. This approach avoids any elaborately designed substrates and holds considerable simplicity and flexibility for reporter design. This study broadens the horizon of the applications of phenanthroline-based transition metal complexes. Furthermore, an efficient and practical method like this has the potential to be widely used in clinical applications and in the point-of-care testing." @default.
- W2328000568 created "2016-06-24" @default.
- W2328000568 creator A5005330529 @default.
- W2328000568 creator A5010717440 @default.
- W2328000568 creator A5058183844 @default.
- W2328000568 date "2010-01-01" @default.
- W2328000568 modified "2023-09-23" @default.
- W2328000568 title "Involvement of glial cells in glucose metabolism in the brain" @default.
- W2328000568 doi "https://doi.org/10.1016/j.neures.2010.07.1046" @default.
- W2328000568 hasPublicationYear "2010" @default.
- W2328000568 type Work @default.
- W2328000568 sameAs 2328000568 @default.
- W2328000568 citedByCount "0" @default.
- W2328000568 crossrefType "journal-article" @default.
- W2328000568 hasAuthorship W2328000568A5005330529 @default.
- W2328000568 hasAuthorship W2328000568A5010717440 @default.
- W2328000568 hasAuthorship W2328000568A5058183844 @default.
- W2328000568 hasConcept C116569031 @default.
- W2328000568 hasConcept C119128265 @default.
- W2328000568 hasConcept C125287762 @default.
- W2328000568 hasConcept C159985019 @default.
- W2328000568 hasConcept C160160445 @default.
- W2328000568 hasConcept C170493617 @default.
- W2328000568 hasConcept C179104552 @default.
- W2328000568 hasConcept C181199279 @default.
- W2328000568 hasConcept C185592680 @default.
- W2328000568 hasConcept C18903297 @default.
- W2328000568 hasConcept C192562407 @default.
- W2328000568 hasConcept C21951064 @default.
- W2328000568 hasConcept C2777289219 @default.
- W2328000568 hasConcept C2777660389 @default.
- W2328000568 hasConcept C2986274086 @default.
- W2328000568 hasConcept C31903555 @default.
- W2328000568 hasConcept C43617362 @default.
- W2328000568 hasConcept C55493867 @default.
- W2328000568 hasConcept C86803240 @default.
- W2328000568 hasConceptScore W2328000568C116569031 @default.
- W2328000568 hasConceptScore W2328000568C119128265 @default.
- W2328000568 hasConceptScore W2328000568C125287762 @default.
- W2328000568 hasConceptScore W2328000568C159985019 @default.
- W2328000568 hasConceptScore W2328000568C160160445 @default.
- W2328000568 hasConceptScore W2328000568C170493617 @default.
- W2328000568 hasConceptScore W2328000568C179104552 @default.
- W2328000568 hasConceptScore W2328000568C181199279 @default.
- W2328000568 hasConceptScore W2328000568C185592680 @default.
- W2328000568 hasConceptScore W2328000568C18903297 @default.
- W2328000568 hasConceptScore W2328000568C192562407 @default.
- W2328000568 hasConceptScore W2328000568C21951064 @default.
- W2328000568 hasConceptScore W2328000568C2777289219 @default.
- W2328000568 hasConceptScore W2328000568C2777660389 @default.
- W2328000568 hasConceptScore W2328000568C2986274086 @default.
- W2328000568 hasConceptScore W2328000568C31903555 @default.
- W2328000568 hasConceptScore W2328000568C43617362 @default.
- W2328000568 hasConceptScore W2328000568C55493867 @default.
- W2328000568 hasConceptScore W2328000568C86803240 @default.
- W2328000568 hasLocation W23280005681 @default.
- W2328000568 hasOpenAccess W2328000568 @default.
- W2328000568 hasPrimaryLocation W23280005681 @default.
- W2328000568 hasRelatedWork W127973334 @default.
- W2328000568 hasRelatedWork W1985945266 @default.
- W2328000568 hasRelatedWork W2023864232 @default.
- W2328000568 hasRelatedWork W2087295519 @default.
- W2328000568 hasRelatedWork W2163896236 @default.
- W2328000568 hasRelatedWork W2884837539 @default.
- W2328000568 hasRelatedWork W2890607849 @default.
- W2328000568 hasRelatedWork W2971208197 @default.
- W2328000568 hasRelatedWork W4308190962 @default.
- W2328000568 hasRelatedWork W4319160411 @default.
- W2328000568 hasVolume "68" @default.
- W2328000568 isParatext "false" @default.
- W2328000568 isRetracted "false" @default.
- W2328000568 magId "2328000568" @default.
- W2328000568 workType "article" @default.