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• W2328061908 abstract "We demonstrate a seamless and contactless method from protein crystallization to X-ray analysis using a microfluidic chip with the aim of obtaining a complete crystallographic data set of a protein crystal under cryogenic conditions. Our microfluidics-based approach did not require direct manipulation of the protein crystal. Therefore, the microfluidic chip approach is suitable for novices of X-ray analysis of protein crystals. We also investigated the effect of stepwise cryoprotection on the quality of protein crystals. Protein crystals with cryoprotection via on-chip manipulation did not show deterioration of crystallographic quality of the protein crystal. The complete diffraction data set of a protein crystal, which is required for determining the 3D structure of the target protein, is obtainable by a simple manipulation." @default.
• W2328061908 created "2016-06-24" @default.
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• W2328061908 date "2015-04-13" @default.
• W2328061908 modified "2023-10-18" @default.
• W2328061908 title "A Method of Cryoprotection for Protein Crystallography by Using a Microfluidic Chip and Its Application for in Situ X-ray Diffraction Measurements" @default.
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• W2328061908 doi "https://doi.org/10.1021/acs.analchem.5b00151" @default.
• W2328061908 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25834918" @default.
• W2328061908 hasPublicationYear "2015" @default.
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