FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2328133634> ?p ?o ?g. }

• W2328133634 abstract "Chlorite dismutase (Cld) and HemQ are structurally and phylogenetically closely related haeme enzymes differing fundamentally in their enzymatic properties. Clds are able to convert chlorite into chloride and dioxygen, whereas HemQ is proposed to be involved in the haeme b synthesis of Gram-positive bacteria. A striking difference between these protein families concerns the proximal haeme cavity architecture. The pronounced H-bonding network in Cld, which includes the proximal ligand histidine and fully conserved glutamate and lysine residues, is missing in HemQ. In order to understand the functional consequences of this clearly evident difference, specific hydrogen bonds in Cld from 'Candidatus Nitrospira defluvii' (NdCld) were disrupted by mutagenesis. The resulting variants (E210A and K141E) were analysed by a broad set of spectroscopic (UV-vis, EPR and resonance Raman), calorimetric and kinetic methods. It is demonstrated that the haeme cavity architecture in these protein families is very susceptible to modification at the proximal site. The observed consequences of such structural variations include a significant decrease in thermal stability and also affinity between haeme b and the protein, a partial collapse of the distal cavity accompanied by an increased percentage of low-spin state for the E210A variant, lowered enzymatic activity concomitant with higher susceptibility to self-inactivation. The high-spin (HS) ligand fluoride is shown to exhibit a stabilizing effect and partially restore wild-type Cld structure and function. The data are discussed with respect to known structure-function relationships of Clds and the proposed function of HemQ as a coprohaeme decarboxylase in the last step of haeme biosynthesis in Firmicutes and Actinobacteria." @default.
• W2328133634 created "2016-06-24" @default.
• W2328133634 creator A5014491856 @default.
• W2328133634 creator A5016279871 @default.
• W2328133634 creator A5016449058 @default.
• W2328133634 creator A5019886162 @default.
• W2328133634 creator A5038728721 @default.
• W2328133634 creator A5040343002 @default.
• W2328133634 creator A5055404869 @default.
• W2328133634 creator A5078523272 @default.
• W2328133634 creator A5082184455 @default.
• W2328133634 creator A5082379253 @default.
• W2328133634 date "2016-03-16" @default.
• W2328133634 modified "2023-10-02" @default.
• W2328133634 title "From chlorite dismutase towards HemQ–the role of the proximal H-bonding network in haeme binding" @default.
• W2328133634 cites W1490646122 @default.
• W2328133634 cites W1494243136 @default.
• W2328133634 cites W1517835039 @default.
• W2328133634 cites W1778245994 @default.
• W2328133634 cites W1932875834 @default.
• W2328133634 cites W1964430082 @default.
• W2328133634 cites W1968418091 @default.
• W2328133634 cites W1968785156 @default.
• W2328133634 cites W1978504467 @default.
• W2328133634 cites W1979820979 @default.
• W2328133634 cites W1981693126 @default.
• W2328133634 cites W1987498087 @default.
• W2328133634 cites W1992346810 @default.
• W2328133634 cites W1996309167 @default.
• W2328133634 cites W1997371123 @default.
• W2328133634 cites W2010474135 @default.
• W2328133634 cites W2014232133 @default.
• W2328133634 cites W2015388145 @default.
• W2328133634 cites W2017147627 @default.
• W2328133634 cites W2021171040 @default.
• W2328133634 cites W2027994152 @default.
• W2328133634 cites W2031108898 @default.
• W2328133634 cites W2031202745 @default.
• W2328133634 cites W2036162627 @default.
• W2328133634 cites W2037899297 @default.
• W2328133634 cites W2041309279 @default.
• W2328133634 cites W2042648353 @default.
• W2328133634 cites W2051864419 @default.
• W2328133634 cites W2055433247 @default.
• W2328133634 cites W2059685195 @default.
• W2328133634 cites W2063368322 @default.
• W2328133634 cites W2064246194 @default.
• W2328133634 cites W2065898375 @default.
• W2328133634 cites W2069471905 @default.
• W2328133634 cites W2075160225 @default.
• W2328133634 cites W2075702811 @default.
• W2328133634 cites W2079263951 @default.
• W2328133634 cites W2079823564 @default.
• W2328133634 cites W2085017321 @default.
• W2328133634 cites W2092383181 @default.
• W2328133634 cites W2092643557 @default.
• W2328133634 cites W2098131246 @default.
• W2328133634 cites W2099389334 @default.
• W2328133634 cites W2111032848 @default.
• W2328133634 cites W2120667954 @default.
• W2328133634 cites W2121233778 @default.
• W2328133634 cites W2124173751 @default.
• W2328133634 cites W2138221558 @default.
• W2328133634 cites W2146051607 @default.
• W2328133634 cites W2159746325 @default.
• W2328133634 cites W2271349249 @default.
• W2328133634 cites W2324254284 @default.
• W2328133634 cites W2328637314 @default.
• W2328133634 cites W2419348636 @default.
• W2328133634 cites W2951542426 @default.
• W2328133634 cites W3004752468 @default.
• W2328133634 cites W917130269 @default.
• W2328133634 doi "https://doi.org/10.1042/bsr20150330" @default.
• W2328133634 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4793301" @default.
• W2328133634 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26858461" @default.
• W2328133634 hasPublicationYear "2016" @default.
• W2328133634 type Work @default.
• W2328133634 sameAs 2328133634 @default.
• W2328133634 citedByCount "22" @default.
• W2328133634 countsByYear W23281336342016 @default.
• W2328133634 countsByYear W23281336342017 @default.
• W2328133634 countsByYear W23281336342018 @default.
• W2328133634 countsByYear W23281336342019 @default.
• W2328133634 countsByYear W23281336342020 @default.
• W2328133634 countsByYear W23281336342021 @default.
• W2328133634 countsByYear W23281336342022 @default.
• W2328133634 countsByYear W23281336342023 @default.
• W2328133634 crossrefType "journal-article" @default.
• W2328133634 hasAuthorship W2328133634A5014491856 @default.
• W2328133634 hasAuthorship W2328133634A5016279871 @default.
• W2328133634 hasAuthorship W2328133634A5016449058 @default.
• W2328133634 hasAuthorship W2328133634A5019886162 @default.
• W2328133634 hasAuthorship W2328133634A5038728721 @default.
• W2328133634 hasAuthorship W2328133634A5040343002 @default.
• W2328133634 hasAuthorship W2328133634A5055404869 @default.
• W2328133634 hasAuthorship W2328133634A5078523272 @default.
• W2328133634 hasAuthorship W2328133634A5082184455 @default.
• W2328133634 hasAuthorship W2328133634A5082379253 @default.
• W2328133634 hasBestOaLocation W23281336341 @default.
• W2328133634 hasConcept C121332964 @default.

• next