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- W2328262006 abstract "Application of the polymerase chain reaction (PCR) method for detection of subgenus B adenoviruses (types 3, 7 and 11) was investigated. It is based on a simple (nonnested) PCR using primer pairs specific for the hexon-coding region. The PCR allowed amplification of DNA from subgenus B adenovirus prototype strains (types 3, 7 and 11) and adenovirus isolates (types 3 and 7), whereas it did not amplify DNA from subgenus A (type 31), C (types 1, 2, 5 and 6), D (types 8, 19 and 37), E (type 4) and adenovirus isolates (types 1, 2, 5 and 6). These results suggest that subgenus B adenoviruses (types 3, 7 and 11) are detectable selectively by means of PCR with primer pairs developed in this study. Amplified fragments from adenovirus types 3, 7 and 11 could be differentiated with restriction endonuclease analysis with Rsa I." @default.
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- W2328262006 date "1998-01-01" @default.
- W2328262006 modified "2023-09-25" @default.
- W2328262006 title "Selective Detection and Differentiation of Subgenus B Adenoviruses (Types 3, 7 and 11) by Polymerase Chain Reaction" @default.
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- W2328262006 doi "https://doi.org/10.11150/kansenshogakuzasshi1970.72.1202" @default.
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