FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2328563325> ?p ?o ?g. }

• W2328563325 endingPage "25" @default.
• W2328563325 startingPage "18" @default.
• W2328563325 abstract "To understand the metabolism of flavonoid rhamnoglycosides by human intestinal microbiota, we measured the metabolic activity of rutin and poncirin (distributed in many functional foods and herbal medicine) by 100 human stool specimens. The average α-Lrhamnosidase activities on the p-nitrophenyl-α-L-rhamnopyranoside, rutin, and poncirin subtrates were 0.10 ± 0.07, 0.25 ± 0.08, and 0.15 ± 0.09 pmol/min/mg, respectively. To investigate the enzymatic properties, α-L-rhamnosidase-producing bacteria were isolated from the specimens, and the α-L-rhamnosidase gene was cloned from a selected organism, Bifidobacterium dentium, and expressed in E. coli. The cloned α-L-rhamnosidase gene contained a 2,673 bp sequcence encoding 890 amino acid residues. The cloned gene was expressed using the pET 26b(+) vector in E. coli BL21, and the expressed enzyme was purified using Ni(2+)-NTA and Q-HP column chromatography. The specific activity of the purified α-L-rhamnosidase was 23.3 μmol/min/mg. Of the tested natural product constituents, the cloned α-L-rhamnosidase hydrolyzed rutin most potently, followed by poncirin, naringin, and ginsenoside Re. However, it was unable to hydrolyze quercitrin. This is the first report describing the cloning, expression, and characterization of α-L-rhamnosidase, a flavonoid rhamnoglycosidemetabolizing enzyme, from bifidobacteria. Based on these findings, the α-L-rhamnosidase of intestinal bacteria such as B. dentium seem to be more effective in hydrolyzing (1-->6) bonds than (1-->2) bonds of rhamnoglycosides, and may play an important role in the metabolism and pharmacological effect of rhamnoglycosides." @default.
• W2328563325 created "2016-06-24" @default.
• W2328563325 creator A5014991511 @default.
• W2328563325 creator A5031730261 @default.
• W2328563325 creator A5059760054 @default.
• W2328563325 creator A5065018052 @default.
• W2328563325 creator A5072339718 @default.
• W2328563325 date "2015-01-28" @default.
• W2328563325 modified "2023-09-30" @default.
• W2328563325 title "Metabolism of Rutin and Poncirin by Human Intestinal Microbiota and Cloning of Their Metabolizing ��-L-Rhamnosidase from Bifidobacterium dentium" @default.
• W2328563325 cites W1895912262 @default.
• W2328563325 cites W1922511005 @default.
• W2328563325 cites W1970462565 @default.
• W2328563325 cites W1974945842 @default.
• W2328563325 cites W1983683309 @default.
• W2328563325 cites W1986863961 @default.
• W2328563325 cites W1992763603 @default.
• W2328563325 cites W2000831478 @default.
• W2328563325 cites W2009301009 @default.
• W2328563325 cites W2014902441 @default.
• W2328563325 cites W2034152261 @default.
• W2328563325 cites W2038975666 @default.
• W2328563325 cites W2047600931 @default.
• W2328563325 cites W2049143523 @default.
• W2328563325 cites W2055321105 @default.
• W2328563325 cites W2058016519 @default.
• W2328563325 cites W2062913054 @default.
• W2328563325 cites W2075499965 @default.
• W2328563325 cites W2078109520 @default.
• W2328563325 cites W2087292184 @default.
• W2328563325 cites W2089623873 @default.
• W2328563325 cites W2108153086 @default.
• W2328563325 cites W2116638825 @default.
• W2328563325 cites W2120929516 @default.
• W2328563325 cites W2121394348 @default.
• W2328563325 cites W2154356891 @default.
• W2328563325 cites W2156530194 @default.
• W2328563325 cites W2230009573 @default.
• W2328563325 doi "https://doi.org/10.4014/jmb.1404.04060" @default.
• W2328563325 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25179902" @default.
• W2328563325 hasPublicationYear "2015" @default.
• W2328563325 type Work @default.
• W2328563325 sameAs 2328563325 @default.
• W2328563325 citedByCount "73" @default.
• W2328563325 countsByYear W23285633252015 @default.
• W2328563325 countsByYear W23285633252016 @default.
• W2328563325 countsByYear W23285633252017 @default.
• W2328563325 countsByYear W23285633252018 @default.
• W2328563325 countsByYear W23285633252019 @default.
• W2328563325 countsByYear W23285633252020 @default.
• W2328563325 countsByYear W23285633252021 @default.
• W2328563325 countsByYear W23285633252022 @default.
• W2328563325 countsByYear W23285633252023 @default.
• W2328563325 crossrefType "journal-article" @default.
• W2328563325 hasAuthorship W2328563325A5014991511 @default.
• W2328563325 hasAuthorship W2328563325A5031730261 @default.
• W2328563325 hasAuthorship W2328563325A5059760054 @default.
• W2328563325 hasAuthorship W2328563325A5065018052 @default.
• W2328563325 hasAuthorship W2328563325A5072339718 @default.
• W2328563325 hasBestOaLocation W23285633251 @default.
• W2328563325 hasConcept C100544194 @default.
• W2328563325 hasConcept C121050878 @default.
• W2328563325 hasConcept C181199279 @default.
• W2328563325 hasConcept C185592680 @default.
• W2328563325 hasConcept C199360897 @default.
• W2328563325 hasConcept C2775963812 @default.
• W2328563325 hasConcept C2778004101 @default.
• W2328563325 hasConcept C2779054382 @default.
• W2328563325 hasConcept C2780206646 @default.
• W2328563325 hasConcept C2780305828 @default.
• W2328563325 hasConcept C41008148 @default.
• W2328563325 hasConcept C523546767 @default.
• W2328563325 hasConcept C54355233 @default.
• W2328563325 hasConcept C55493867 @default.
• W2328563325 hasConcept C86803240 @default.
• W2328563325 hasConcept C89423630 @default.
• W2328563325 hasConceptScore W2328563325C100544194 @default.
• W2328563325 hasConceptScore W2328563325C121050878 @default.
• W2328563325 hasConceptScore W2328563325C181199279 @default.
• W2328563325 hasConceptScore W2328563325C185592680 @default.
• W2328563325 hasConceptScore W2328563325C199360897 @default.
• W2328563325 hasConceptScore W2328563325C2775963812 @default.
• W2328563325 hasConceptScore W2328563325C2778004101 @default.
• W2328563325 hasConceptScore W2328563325C2779054382 @default.
• W2328563325 hasConceptScore W2328563325C2780206646 @default.
• W2328563325 hasConceptScore W2328563325C2780305828 @default.
• W2328563325 hasConceptScore W2328563325C41008148 @default.
• W2328563325 hasConceptScore W2328563325C523546767 @default.
• W2328563325 hasConceptScore W2328563325C54355233 @default.
• W2328563325 hasConceptScore W2328563325C55493867 @default.
• W2328563325 hasConceptScore W2328563325C86803240 @default.
• W2328563325 hasConceptScore W2328563325C89423630 @default.
• W2328563325 hasIssue "1" @default.
• W2328563325 hasLocation W23285633251 @default.
• W2328563325 hasLocation W23285633252 @default.
• W2328563325 hasOpenAccess W2328563325 @default.
• W2328563325 hasPrimaryLocation W23285633251 @default.
• W2328563325 hasRelatedWork W1963705127 @default.

• next