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- W2328571099 abstract "A novel series of glycolipids containing cholinephosphoryl (→6) -N-acetylglucosamine as an amphoteric group was found present in whole tissues of the porcine parasitic nematode, Ascaris suum. Thin layer chromatography of the total amphoteric glycolipids indicated at least seven components to react positively with orcinol-sulfuric acid (hexose), Hanes-Isherwood (phosphate) and Dragendorff (choline) spray reagents. Four of these components (PGL1PGL4) were purified and their chemical structures completely characterized as PGL1 : cholinephosphoryl→6GlcNAcβ1-3Manβ1-4Glcβ1-Cer, PGL2 : GalNAcβ1-4 (cholinephosphoryl→6) GlcNAcβ1-3Manβ1-4Glcβ1-Cer, PGL3 : Galα1-3GalNAcβ1-4 (cholinephosphoryl→6) GlcNAcβ1-3Manβ1-4Glcβ1-Cer and PGL4 : a mixture of Galβ1-3Galα1-3GalNAcβ1-4 (cholinephosphoryl→6) GlcNAcβ1-3Manβ1-4Glcβ1-Cer and Galβ1-6Galα1-3GalNAcβ1-4 (cholinephosphoryl→6) GlcNAcβ1-3Manβ1-4Glcβ1-Cer. All characterizations were based on compositional sugar, fatty acid and sphingoid analyses, hydrogen fluoride degradation, acid hydrolysis, methylation analysis, exoglycosidase degradation, thin layer chromatography-immunostaining and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The ceramide moiety was found to consist of 2-hydroxydocosanoic acid and 2-hydroxytetracosanoic acid as the major fatty acids, and 15-methylhexadecasphing-4-enine and 15-methylhexadecasphinganine as the main sphingoids." @default.
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- W2328571099 date "2000-01-01" @default.
- W2328571099 modified "2023-10-02" @default.
- W2328571099 title "Amphoteric Glycosphingolipids in Parasitic Nematode (I) :" @default.
- W2328571099 doi "https://doi.org/10.5650/jos1996.49.127" @default.
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