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- W2328851838 abstract "Metaproteomics has a bright future for its broad application in understanding the interaction between environment and function,metabolism of the soil microbial community.Due to the specialty of soil sample,the extraction method is one of the main bottlenecks which limit their wide application.This paper summarized research actuality of soil protein extraction on several aspects from sample preparation,extraction methods and influencing factors,pointed out some currently-existing research problems,and put forward new extraction method and thought exploiture from traditional methods.In general,only by proceeding from the experimental purpose,protein types and follow-up study,setting up the corresponding extraction strategy,can the extraction problem of soil protein be effectively solved.According to the source and distribution,the soil protein can be divided into three types,total protein,extracellular protein and intracellular protein,thus the extraction method of three types of protein are different from each other.The direct extraction method is mostly used in the total protein extraction;the extraction method of extracellular protein does not require lysis;the intracellular protein can be extracted by direct or indirect methods and so on.The extract yield can be affected by the methods of lysis,concentration and removal of humus,as well as the type and pH value of the extraction buffer.In addition,the application prospects of the soil metaproteome and future research work were also discussed.Tab 1,Ref 36" @default.
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- W2328851838 date "2012-01-01" @default.
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- W2328851838 title "Methods of Protein Extraction from Soil and Their Potential Application inStudy of Soil Metaproteome" @default.
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- W2328851838 doi "https://doi.org/10.3724/sp.j.1145.2012.00691" @default.
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