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• W2328858206 abstract "Event Abstract Back to Event The study of genes encoding interleukin-1β, 2, 8,10 expression in the rabbit infected with RHD (rabbit haemorrhagic disease). Alicja Trzeciak-Ryczek1, Beata Tokarz-Deptuła1 and Wiesław Deptuła2* 1 University of Szczecin, Faculty of Biology, Department of Immunology, Poland 2 University of Szczecin, Faculty of Biology, Department of Microbiology , Poland Interleukins (IL) play an important role in antiviral immunity, for example in infection of rabbits with RHDV, which is an etiological factor of viral haemorrhagic disease. Studies on the changes in the expression of genes encoding IL are few, and applies only to liver cells. Thus, the aim was to assess changes in the expression of genes encoding IL-1β, 2,8,10 in peripheral blood leukocytes (components of the immune system) in rabbits experimentally infected with RHD virus- strain V-411. The analysis was performed with real-time PCR, and the results were analyzed by relative quantification method by Livak, using two reference genes (HPRT and β-actin). The study was performed on 6 rabbits (4 individuals infected intramuscularly with RHDV and 2 control) and the blood was sampled from ear’s marginal vein in 0 (ie. before administration of antigen and placebo), and then in 8,12,24,28,36 p.i. Studies have shown the increased expression of the genes encoding IL-2 in the 8 and 12 h p.i. and decrease in the 24-36h p.i. A similar, but not identical picture of changes was registered in the expression of gene for IL 1β. For IL-10, the decrease of expression was registered in 8 and 12h p.i. and the increase between 24-36h. There have been no changes in the expression of genes encoding IL-8 registered. Keywords: Interleukins, cytokines/chemokines, Gene Expression, Real-Time PCR, RHD Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Trzeciak-Ryczek A, Tokarz-Deptuła B and Deptuła W (2013). The study of genes encoding interleukin-1β, 2, 8,10 expression in the rabbit infected with RHD (rabbit haemorrhagic disease).. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.01090 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 11 Jul 2013; Published Online: 22 Aug 2013. * Correspondence: Prof. Wiesław Deptuła, University of Szczecin, Faculty of Biology, Department of Microbiology, Szczecin, 71-412, Poland, kurp13@univ.szczecin.pl Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Alicja Trzeciak-Ryczek Beata Tokarz-Deptuła Wiesław Deptuła Google Alicja Trzeciak-Ryczek Beata Tokarz-Deptuła Wiesław Deptuła Google Scholar Alicja Trzeciak-Ryczek Beata Tokarz-Deptuła Wiesław Deptuła PubMed Alicja Trzeciak-Ryczek Beata Tokarz-Deptuła Wiesław Deptuła Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page." @default.
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• W2328858206 title "The study of genes encoding interleukin-1�, 2, 8,10 expression in the rabbit infected with RHD (rabbit haemorrhagic disease)." @default.
• W2328858206 doi "https://doi.org/10.3389/conf.fimmu.2013.02.01090" @default.
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