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- W2328874037 abstract "Cingulin (CGN) is a Mr 140 kDa protein, which is localized in the cytoplasmic region of vertebrate tight junctions (TJ), and regulates gene expression and RhoA signalling in cultured cells. To investigate the function of CGN at the organism level, we generated CGN knockout (CGN−/−) mice by homologous recombination. CGN−/− mice are viable and fertile, and are born at the expected mendelian ratios. Immunohistochemistry, immunofluorescence, electron microscopy, and permeability assays of epithelial tissues of CGN−/− mice show no cingulin labelling at junctions, normal localization of TJ proteins, and normal TJ structure and barrier function. Microarray analysis of intestinal cells does not show significant changes in gene expression between CGN−/− and CGN+/+ mice, whereas immunoblotting analysis shows a 2-fold increase in the levels of claudin-2 protein in the duodenum and the kidney of CGN−/− mice, compared to CGN+/+ littermates. Furthermore, CGN−/− mice show an exacerbated response to the ulcerogenic action of cysteamine, whereas acute injury of the colon by dextran sodium sulphate elicits undistinguishable responses in CGN−/− and CGN+/+ mice. We conclude that at the organism level cingulin is dispensable for the structure and barrier function of TJ, and it is embedded in signalling networks that control the expression of claudin-2, and the mucosal response to acute injury in the duodenum." @default.
- W2328874037 created "2016-06-24" @default.
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- W2328874037 date "2012-01-01" @default.
- W2328874037 modified "2023-10-18" @default.
- W2328874037 title "Cingulin is dispensable for epithelial barrier function and tight junction structure, and plays a role in the control of claudin-2 expression and response to duodenal mucosa injury" @default.
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- W2328874037 doi "https://doi.org/10.1242/jcs.101261" @default.
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