FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2329620766> ?p ?o ?g. }

• W2329620766 endingPage "2712" @default.
• W2329620766 startingPage "2708" @default.
• W2329620766 abstract "A competitive immunoassay based on chemiluminescence resonance energy transfer (CRET) on the magnetic beads (MBs) is developed for the detection of human immunoglobulin G (IgG). In this protocol, carboxyl-modified MBs were conjugated with horseradish peroxidase (HRP)-labeled goat antihuman IgG (HRP-anti-IgG) and incubated with a limited amount of fluorescein isothiocyanate (FITC)-labeled human IgG to immobilize the antibody–antigen immune complex on the surface of the MBs, which was further incubated with the target analyte (human IgG) for competitive immunoreaction and separated magnetically to remove the supernatant. The chemiluminescence (CL) buffer (containing luminol and H2O2) was then added, and the CRET from donor luminol to acceptor FITC in the immunocomplex on the surface of MBs occured immediately. The present protocol was evaluated for the competitive immunoassay of human IgG, and a linear relationship between CL intensity ratio (R = I425/I525) and human IgG concentration in the range of 0.2–4.0 nM was obtained with a correlation coefficient of 0.9965. The regression equation was expressed as R = 1.9871C + 2.4616, and a detection limit of 2.9 × 10–11 M was obtained. The present method was successfully applied for the detection of IgG in human serum. The results indicate that the present protocol is quite promising for the application of CRET in immunoassays. It could also be developed for detection of other antigen–antibody immune complexes by using the corresponding antigens and respective antibodies." @default.
• W2329620766 created "2016-06-24" @default.
• W2329620766 creator A5013307481 @default.
• W2329620766 creator A5015061587 @default.
• W2329620766 creator A5056353229 @default.
• W2329620766 creator A5083385082 @default.
• W2329620766 creator A5090037751 @default.
• W2329620766 date "2012-02-28" @default.
• W2329620766 modified "2023-09-25" @default.
• W2329620766 title "Magnetic Bead-Sensing-Platform-Based Chemiluminescence Resonance Energy Transfer and Its Immunoassay Application" @default.
• W2329620766 cites W1916948239 @default.
• W2329620766 cites W1967738476 @default.
• W2329620766 cites W1976292255 @default.
• W2329620766 cites W1983670987 @default.
• W2329620766 cites W1984701286 @default.
• W2329620766 cites W1986308794 @default.
• W2329620766 cites W1992555864 @default.
• W2329620766 cites W1994204120 @default.
• W2329620766 cites W2005390361 @default.
• W2329620766 cites W2017248460 @default.
• W2329620766 cites W2041505869 @default.
• W2329620766 cites W2049636134 @default.
• W2329620766 cites W2053389193 @default.
• W2329620766 cites W2056001141 @default.
• W2329620766 cites W2075249985 @default.
• W2329620766 cites W2086748420 @default.
• W2329620766 cites W2088032311 @default.
• W2329620766 cites W2094478666 @default.
• W2329620766 cites W2100285343 @default.
• W2329620766 cites W2129482865 @default.
• W2329620766 cites W2142844453 @default.
• W2329620766 cites W2156245425 @default.
• W2329620766 cites W2330004524 @default.
• W2329620766 cites W4230860477 @default.
• W2329620766 doi "https://doi.org/10.1021/ac202959d" @default.
• W2329620766 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22339489" @default.
• W2329620766 hasPublicationYear "2012" @default.
• W2329620766 type Work @default.
• W2329620766 sameAs 2329620766 @default.
• W2329620766 citedByCount "64" @default.
• W2329620766 countsByYear W23296207662012 @default.
• W2329620766 countsByYear W23296207662013 @default.
• W2329620766 countsByYear W23296207662014 @default.
• W2329620766 countsByYear W23296207662015 @default.
• W2329620766 countsByYear W23296207662016 @default.
• W2329620766 countsByYear W23296207662017 @default.
• W2329620766 countsByYear W23296207662018 @default.
• W2329620766 countsByYear W23296207662019 @default.
• W2329620766 countsByYear W23296207662020 @default.
• W2329620766 countsByYear W23296207662021 @default.
• W2329620766 countsByYear W23296207662022 @default.
• W2329620766 countsByYear W23296207662023 @default.
• W2329620766 crossrefType "journal-article" @default.
• W2329620766 hasAuthorship W2329620766A5013307481 @default.
• W2329620766 hasAuthorship W2329620766A5015061587 @default.
• W2329620766 hasAuthorship W2329620766A5056353229 @default.
• W2329620766 hasAuthorship W2329620766A5083385082 @default.
• W2329620766 hasAuthorship W2329620766A5090037751 @default.
• W2329620766 hasConcept C10390740 @default.
• W2329620766 hasConcept C119128265 @default.
• W2329620766 hasConcept C121332964 @default.
• W2329620766 hasConcept C145802702 @default.
• W2329620766 hasConcept C147483822 @default.
• W2329620766 hasConcept C159654299 @default.
• W2329620766 hasConcept C181199279 @default.
• W2329620766 hasConcept C185592680 @default.
• W2329620766 hasConcept C203014093 @default.
• W2329620766 hasConcept C2776316542 @default.
• W2329620766 hasConcept C2777019933 @default.
• W2329620766 hasConcept C2777676563 @default.
• W2329620766 hasConcept C2780420688 @default.
• W2329620766 hasConcept C2780898057 @default.
• W2329620766 hasConcept C43617362 @default.
• W2329620766 hasConcept C55493867 @default.
• W2329620766 hasConcept C62520636 @default.
• W2329620766 hasConcept C86803240 @default.
• W2329620766 hasConcept C91881484 @default.
• W2329620766 hasConceptScore W2329620766C10390740 @default.
• W2329620766 hasConceptScore W2329620766C119128265 @default.
• W2329620766 hasConceptScore W2329620766C121332964 @default.
• W2329620766 hasConceptScore W2329620766C145802702 @default.
• W2329620766 hasConceptScore W2329620766C147483822 @default.
• W2329620766 hasConceptScore W2329620766C159654299 @default.
• W2329620766 hasConceptScore W2329620766C181199279 @default.
• W2329620766 hasConceptScore W2329620766C185592680 @default.
• W2329620766 hasConceptScore W2329620766C203014093 @default.
• W2329620766 hasConceptScore W2329620766C2776316542 @default.
• W2329620766 hasConceptScore W2329620766C2777019933 @default.
• W2329620766 hasConceptScore W2329620766C2777676563 @default.
• W2329620766 hasConceptScore W2329620766C2780420688 @default.
• W2329620766 hasConceptScore W2329620766C2780898057 @default.
• W2329620766 hasConceptScore W2329620766C43617362 @default.
• W2329620766 hasConceptScore W2329620766C55493867 @default.
• W2329620766 hasConceptScore W2329620766C62520636 @default.
• W2329620766 hasConceptScore W2329620766C86803240 @default.
• W2329620766 hasConceptScore W2329620766C91881484 @default.
• W2329620766 hasIssue "6" @default.
• W2329620766 hasLocation W23296207661 @default.

• next