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- W2329667928 abstract "Balb/c mice were injected with preparations of a sex-limited esterase (Est-m) produced in the accessory glands of adult male western corn rootworms, Diabrotica virgifera virgifera LeConte. Hybridoma technology was then used in conjunction with a plate ELISA to recover an array of 15 monoclonal antibodies (Mab) reactive against the native enzyme. Isotyping showed that all 15 Mab were of the IgM class. Seven Mab variously failed to react in dot blot or western blot assays directed against native or denatured Est-m, respectively. A I»gtll cDNA expression library was generated from accessory gland mRNA and screened for the production of potential Est-m protein sequences using a cocktail of six generally reactive Mab. Seven positive clones were recovered and retested against a panel of 12 individual Mab after amplification." @default.
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- W2329667928 date "1992-07-01" @default.
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- W2329667928 title "Monoclonal Antibodies Against Est-m of Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae): Recovery, Characterization, and Use in Immunoassays of a cDNA Expression Library" @default.
- W2329667928 doi "https://doi.org/10.1093/aesa/85.4.499" @default.
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