FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2329838155> ?p ?o ?g. }

• W2329838155 endingPage "116" @default.
• W2329838155 startingPage "108" @default.
• W2329838155 abstract "The aim of this study was to further our understanding of the transformation process by identifying differentially expressed proteins in melanocytes compared with melanoma cell lines. Tandem mass spectrometry incorporating iTRAQ reagents was used as a screen to identify and comparatively quantify the expression of proteins in membrane-enriched samples isolated from primary human melanocytes or three melanoma cells lines. Real-time PCR was used to validate significant hits. Immunohistochemistry was used to validate the expression of proteins of interest in melanocytes in human skin and in melanoma-infiltrated lymph nodes. Publically available databases were examined to assess mRNA expression and correlation to patient outcome in a larger cohort of samples. Finally, preliminary functional studies were carried out using siRNAs to reduce the expression of a protein of interest in primary melanocytes and in a keratinocyte cell line. Two proteins, AHNAK and ANXA2, were significantly downregulated in the melanoma cell lines compared with melanocytes. Downregulation was confirmed in tumor cells in a subset of human melanoma-infiltrated human lymph nodes compared with melanocytes in human skin. Examination of Gene Expression Omnibus database data sets suggests that downregulation of AHNAK mRNA and mutation of the AHNAK gene are common in metastatic melanoma and correlates to a poor outcome. Knockdown of AHNAK in primary melanocytes and in a keratinocyte cell line led to a reduction in detectable cadherin-1. This is the first report that we are aware of which correlates a loss of AHNAK with melanoma and poor patient outcome. We hypothesize that AHNAK is required for the expression of functional cadherin-1." @default.
• W2329838155 created "2016-06-24" @default.
• W2329838155 creator A5022058325 @default.
• W2329838155 creator A5044589609 @default.
• W2329838155 creator A5056824555 @default.
• W2329838155 creator A5079814428 @default.
• W2329838155 creator A5080681131 @default.
• W2329838155 date "2016-04-01" @default.
• W2329838155 modified "2023-09-23" @default.
• W2329838155 title "AHNAK is downregulated in melanoma, predicts poor outcome, and may be required for the expression of functional cadherin-1" @default.
• W2329838155 cites W1932933996 @default.
• W2329838155 cites W1964490219 @default.
• W2329838155 cites W1976546353 @default.
• W2329838155 cites W1977852460 @default.
• W2329838155 cites W1981148075 @default.
• W2329838155 cites W1984759958 @default.
• W2329838155 cites W1986914055 @default.
• W2329838155 cites W1993573257 @default.
• W2329838155 cites W1996067072 @default.
• W2329838155 cites W2002712251 @default.
• W2329838155 cites W2003886366 @default.
• W2329838155 cites W2006398015 @default.
• W2329838155 cites W2009144650 @default.
• W2329838155 cites W2012449208 @default.
• W2329838155 cites W2014504800 @default.
• W2329838155 cites W2016701131 @default.
• W2329838155 cites W2018244760 @default.
• W2329838155 cites W2026462337 @default.
• W2329838155 cites W2027359413 @default.
• W2329838155 cites W2027821912 @default.
• W2329838155 cites W2028314782 @default.
• W2329838155 cites W2030090912 @default.
• W2329838155 cites W2045894877 @default.
• W2329838155 cites W2054531472 @default.
• W2329838155 cites W2056578809 @default.
• W2329838155 cites W2063392612 @default.
• W2329838155 cites W2065723650 @default.
• W2329838155 cites W2065832539 @default.
• W2329838155 cites W2073078086 @default.
• W2329838155 cites W2077178117 @default.
• W2329838155 cites W2079818771 @default.
• W2329838155 cites W2088662406 @default.
• W2329838155 cites W2088783764 @default.
• W2329838155 cites W2101666731 @default.
• W2329838155 cites W2107914050 @default.
• W2329838155 cites W2109972881 @default.
• W2329838155 cites W2117692326 @default.
• W2329838155 cites W2134471382 @default.
• W2329838155 cites W2148317031 @default.
• W2329838155 cites W2148489676 @default.
• W2329838155 cites W2155384606 @default.
• W2329838155 cites W2160095726 @default.
• W2329838155 cites W2173336042 @default.
• W2329838155 cites W2415239332 @default.
• W2329838155 cites W4234329228 @default.
• W2329838155 doi "https://doi.org/10.1097/cmr.0000000000000228" @default.
• W2329838155 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4777222" @default.
• W2329838155 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26672724" @default.
• W2329838155 hasPublicationYear "2016" @default.
• W2329838155 type Work @default.
• W2329838155 sameAs 2329838155 @default.
• W2329838155 citedByCount "29" @default.
• W2329838155 countsByYear W23298381552016 @default.
• W2329838155 countsByYear W23298381552017 @default.
• W2329838155 countsByYear W23298381552018 @default.
• W2329838155 countsByYear W23298381552019 @default.
• W2329838155 countsByYear W23298381552020 @default.
• W2329838155 countsByYear W23298381552021 @default.
• W2329838155 countsByYear W23298381552022 @default.
• W2329838155 countsByYear W23298381552023 @default.
• W2329838155 crossrefType "journal-article" @default.
• W2329838155 hasAuthorship W2329838155A5022058325 @default.
• W2329838155 hasAuthorship W2329838155A5044589609 @default.
• W2329838155 hasAuthorship W2329838155A5056824555 @default.
• W2329838155 hasAuthorship W2329838155A5079814428 @default.
• W2329838155 hasAuthorship W2329838155A5080681131 @default.
• W2329838155 hasBestOaLocation W23298381552 @default.
• W2329838155 hasConcept C104317684 @default.
• W2329838155 hasConcept C127561419 @default.
• W2329838155 hasConcept C127716648 @default.
• W2329838155 hasConcept C1491633281 @default.
• W2329838155 hasConcept C149402561 @default.
• W2329838155 hasConcept C173396325 @default.
• W2329838155 hasConcept C203014093 @default.
• W2329838155 hasConcept C204232928 @default.
• W2329838155 hasConcept C2777074287 @default.
• W2329838155 hasConcept C2777658100 @default.
• W2329838155 hasConcept C2779769559 @default.
• W2329838155 hasConcept C2780932548 @default.
• W2329838155 hasConcept C502942594 @default.
• W2329838155 hasConcept C54355233 @default.
• W2329838155 hasConcept C81885089 @default.
• W2329838155 hasConcept C86803240 @default.
• W2329838155 hasConceptScore W2329838155C104317684 @default.
• W2329838155 hasConceptScore W2329838155C127561419 @default.
• W2329838155 hasConceptScore W2329838155C127716648 @default.
• W2329838155 hasConceptScore W2329838155C1491633281 @default.
• W2329838155 hasConceptScore W2329838155C149402561 @default.

• next