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• W2329986137 abstract "Event Abstract Back to Event The control of mesenchymal stem cell differentiation using matrix and induction media Jing He1, Bo Jiang1, Ruijuan Yao1, Yao Wu1 and Fang Wu1 1 Sichuan University, National Engineering Research Center for Biomaterials, China Introduction: MSC functions and cell fate have been affected by its microenvironments, including chemical environment (soluble and insoluble factors[1], surface chemistry[2] etc.) and physical environment (matrix elasticity, mechanical force[3]).While both induction culture media and matrix have been reported to regulate the stem cell fate, little is known about which factor plays a more decisive role in directing the MSC differentiation lineage as well as the underlying mechanisms. To this aim, we performed a systematic comparison of the effect of induction media and matrix selection (HA-collagen and HA-synthetic hydrogel) on MSC adhesion, cytoskeleton organization, proliferation, and differentiation. Materials and methods: Porous hydroxyapatite coatings were prepared through a novel liquid precursor plasma spraying process and infiltrated with the collagen and synthetic hydrogel, respectively[4]. Suspension of MSCs was seeded on HA-collagen and HA-synthetic hydrogel substrate surfaces, and was cultured with the osteogenic induction media, chondrogenic induction media and normal culture media, respectively. A systematic comparison has been carried out on the effects of induction media and matrix on MSC adhesion, cytoskeleton organization, proliferation, and differentiation, in particular the transcriptional activities leading to the osteoblastic and chondrocytic lineages. Result and discussion: For osteoblastic differentiation, the selection of HA-collagen matrix over the HA-synthetic hydrogel significantly increased the ALP and OCN expressions regardless of the culture condition, whereas the osteogenic induction media only moderately increased the ALP activity and showed no effect on the OCN secretion (Fig. 1). On the other hand, the HA-synthetic hydrogel matrix strongly favored the chondrocytic differentiation regardless of the culture conditions, showing an upward trend for Col II and significantly up-regulation of Col X under all three culture media. The chondrogenic induction media however had almost no effect on Col II secretion and moderately increased the Col X secretion for HA-collagen matrix alone (Fig. 2). The results clearly suggested a striking role of matrix/substrate in directing the MSC differentiation lineage. Then we further exploited the likely mechanisms that led to the strong modulation effect of matrix on the transcriptional activities. We examined the Wnt-1 and β-Catenin, two key markers in the canonical Wnt-β-Catenin signal pathway, which plays a critical role in mechnaotransduction and in activation of the osteoblastic differentiation. The results showed that the HA-collagen matrix had strong stimulation effect on the activation of the Wnt-β-Catenin signal pathway, by significantly up-regulating the expressions of Wnt-1 and β-Catenin, where only minor influence was observed for induction media. Conclusion: Overall, our results demonstrated that the matrix selection had a much more profound effect on directing the differentiation lineage than the induction media did. The strong modulation effect on the transcription activities from the matrix might be the critical factor contributing to such events, where canonical Wnt-β-Catenin signal pathway was directly involved in the matrix-driven osteoblastic differentiation. Such findings not only provide a critical insight on natural cellular events leading to the osteoblastic and chondrocytic differentiations, but also have important implications in biomaterial design for tissue engineering applications such as osteochondral constructs. Fig. 1. Osteoblastic differentiation Fig. 2. Chondrocytic differentiation References:[1] VH. Fan, et al., Stem Cells, 2007, 25, 1241;[2] DSW. Benoit, et al., Nature materials, 2008, 7, 816[3] S. Even-Ram, et al., Cell, 2006, 126, 645[4] J. He, et al., Biomaterials, 2013, 34, 6580 Keywords: Cell Differentiation, Extracellular Matrix, stem cell, matrix-cell interaction Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: General Session Oral Topic: Biomaterials and cellular signaling Citation: He J, Jiang B, Yao R, Wu Y and Wu F (2016). The control of mesenchymal stem cell differentiation using matrix and induction media. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02788 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Jing He Bo Jiang Ruijuan Yao Yao Wu Fang Wu Google Jing He Bo Jiang Ruijuan Yao Yao Wu Fang Wu Google Scholar Jing He Bo Jiang Ruijuan Yao Yao Wu Fang Wu PubMed Jing He Bo Jiang Ruijuan Yao Yao Wu Fang Wu Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. 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• W2329986137 title "The control of mesenchymal stem cell differentiation using matrix and induction media" @default.
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