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- W2330304874 abstract "We analyzed the light requirements for celery somatic embryogenesis and plantlet development. The regeneration period was divided into two stages. During the first stage, in which torpedo-shaped embryos were mainly produced from the embryogenic callus, light stimulated chlorophyll synthesis. Although under dark conditions cells were slow in embryo formation, prolonged culture in the dark led to the production of globular-, heart- and torpedo-shaped embryos. The torpedo embryos were ready to become healthy plantlets after the second-stage culture under light conditions. Light enhanced the embryo formation rate, but was not obligatory on embryo formation. In the second stage, a 16-h photoperiod and a higher light intensity gave the maximum frequency of Plantlet development, and were essential for the production of the healthy plantlets in terms of size (more than 850μm). The results strongly suggest that illumination can be reduced at the beginning of the first regeneration stage, but that increased illumination is required after the torpedo embryo development has progressed." @default.
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- W2330304874 date "1993-01-01" @default.
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- W2330304874 title "Light Dependency in Celery Somatic Embryogenesis and Plantlet Development in Suspension Culture." @default.
- W2330304874 doi "https://doi.org/10.5511/plantbiotechnology1984.10.25" @default.
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