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- W2330394502 abstract "Superquenching of aggregation-induced emission (AIE) has been utilized in biosensing for the first time. A positively charged tetraphenylethene derivative (compound 1) showed no emission in an aqueous buffer solution. A single-stranded DNA (a polyanion) induced aggregation of compound 1, and strong compound 1 aggregate emission was observed. When the DNA was labeled with a quencher molecule, compound 1 aggregate emission was efficiently quenched. On the basis of this observation, a new, simple, sensitive and selective DNA methyltransferase (MTase) assay has been developed. A quencher-labeled double-stranded DNA could induce aggregation of compound 1, and superquenching of compound 1 AIE was observed. In the presence of MTase and an endonuclease, the DNA could be specifically methylated and cleaved into single-stranded DNA fragments. The quencher molecule was released, and a turn-on emission signal was detected." @default.
- W2330394502 created "2016-06-24" @default.
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- W2330394502 date "2014-09-22" @default.
- W2330394502 modified "2023-10-17" @default.
- W2330394502 title "Nucleic Acid-Induced Tetraphenylethene Probe Noncovalent Self-Assembly and the Superquenching of Aggregation-Induced Emission" @default.
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- W2330394502 doi "https://doi.org/10.1021/ac502496h" @default.
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