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- W2330528097 abstract "The community structure of cultivable bacteria associated with the marine sponge, Petrosia corticata, collected from Jeju Island in summer (September) of 2012 and winter (January) of 2013, were compared by the PCR-ARDRA method. Bacterial strains were cultured for 4 days at <TEX>$26^{circ}C$</TEX> on Zobell medium and marine agar medium. After PCR amplification of 16S rRNA gene of individual strains, the restriction enzymes MspI and HaeIII were used to make restriction patterns. As a result, 24 ARDRA patterns from the summer sponge and 20 ARDRA patterns from the winter sponge were obtained. The sequencing result of 1-3 selected strains from each pattern showed over 98% similarities with the known sequences from the public database. At the phylum level, the bacterial community structures of both sponges (summer and winter) were identical qualitatively and composed of 4 phyla : Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes. Alphaproteobacteria accounted for 42.5% of total in summer sponge and 25.2% in winter, decreasing in the winter sample. Gammaproteobacteria accounted for 27.5% of total in summer sponge and 35.2% in winter, increasing in the winter sample. At the genus and species level, summer sponge had more diverse bacterial communities than winter sponge. Actinobacteria, Bacteroidetes, and Firmicutes increased in the winter sample." @default.
- W2330528097 created "2016-06-24" @default.
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- W2330528097 date "2015-12-31" @default.
- W2330528097 modified "2023-10-14" @default.
- W2330528097 title "Seasonal Differences of Cultivable Bacterial Communities Associated with the Marine Sponge, Petrosia corticata, Collected from Jeju Island" @default.
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- W2330528097 doi "https://doi.org/10.15433/ksmb.2015.7.2.042" @default.
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