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• W2330544384 abstract "For more than a decade, the backfilling approach for the immobilization of DNA probes has been routinely adopted for the construction of functional interfaces; however, reliably reproducing electrochemical signal amplification by this method is a challenge. In this research, we demonstrate that the insertion approach significantly bolsters the reproducibility of electrochemical signal amplification via DNA superstructures. The combination of the backfilling approach and the DNA superstructure formation poses a big challenge to reliably reproducing electrochemical signal amplification. In order to use the detection of Hg2+ as a prototype of this new strategy, a thymine-rich DNA probe that is specific to mercury ion was applied in this study. The presence of Hg2+ induces the folding of the DNA probes and inhibits the formation of DNA superstructures. By using electroactive probes ([Ru(NH3)6]3+) that are electrostatically adsorbed onto the double strands, differential pulse voltammetry (DPV) could quantitatively confirm the presence of Hg2+. A limit of detection (LOD) and a limit of quantification (LOQ) (LOQ) as low as 0.3 and 9.5 pM, respectively, were achieved. Furthermore, excellent selectivity and real sample analysis demonstrated the promising potential of this approach in future applications." @default.
• W2330544384 created "2016-06-24" @default.
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• W2330544384 date "2014-05-02" @default.
• W2330544384 modified "2023-09-30" @default.
• W2330544384 title "Insertion Approach: Bolstering the Reproducibility of Electrochemical Signal Amplification via DNA Superstructures" @default.
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• W2330544384 doi "https://doi.org/10.1021/ac500029z" @default.
• W2330544384 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24761933" @default.
• W2330544384 hasPublicationYear "2014" @default.
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