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- W2330723090 abstract "A micromethod is described that uses whole blood for studying mitogen stimulation of chicken lymphocytes. Procedures critical for obtaining a stimulatory response up to 200 times that of unstimulated control cultures include: 1) use of no more than 10 microliter of whole blood per culture; 2) incubation at 41 C for 72 hours in 0.2 ml of medium RPMI 1640 containing 100 microgram of stock phytohemagglutinin-P and 3) pulse labeling for 8 hours with 5-[125I] iodo-2'-deoxyruidine. The method is easy, rapid, and suitable for sequential studies of pathogenesis of disease in poultry." @default.
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- W2330723090 date "1978-04-01" @default.
- W2330723090 modified "2023-09-26" @default.
- W2330723090 title "Chicken Lymphocyte Stimulation by Mitogens: A Microassay with Whole-Blood Cultures" @default.
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- W2330723090 doi "https://doi.org/10.2307/1589541" @default.
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