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• W2330778891 abstract "Although microRNAs (miRNAs) have been implicated in fine-tuning gene networks, the roles of mmu- mir-143 (miR-143) in mammalian ovary development have not been studied in vitro. We investigated the expression and function of miR-143 in the mouse ovary during primordial follicle formation. Real-time polymerase chain reaction analysis showed that miR-143 expression increased during primordial follicle formation from 15.5 days post-coitus to 4 days post-partum. miR-143 was located in pregranulosa cells by in situ hybridization. To study the function of miR-143 in primordial follicle formation we established an electroporation transfection model in vitro that allowed miR-143 expression to be efficiently upregulated and inhibited in cultured ovaries. Further studies showed that miR-143 inhibited the formation of primordial follicles by suppressing pregranulosa cell proliferation and downregulating the expression of genes related to the cell cycle. These findings suggest that miR-143 is critical for the formation of primordial follicles and regulates ovarian development and function." @default.
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• W2330778891 date "2013-01-01" @default.
• W2330778891 modified "2023-10-16" @default.
• W2330778891 title "miR-143 is critical for the formation of primordial follicles in mice " @default.
• W2330778891 doi "https://doi.org/10.2741/4122" @default.
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