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- W2330791323 abstract "The pathways involved in the persistence of chronic colitis in the murine chronic colitis model of dextran sulfate sodium (DSS) induced colitis are uncertain. The physiology in the late phase of colitis may mimic chronic inflammatory bowel disease. The purpose of our study was to identify signature differences in mucosal inflammatory gene expression between acute and chronic colitis in a cycled DSS mouse model. Balb/cJ mice were subjected to three successive cycles of 4% DSS in drinking water (5 days on/7 days off) followed by regular drinking water up to 50 days. Mice were sacrificed in groups of 3 along with eight controls on days 5, 12, 24, 36, 43, and 50. Colitis activity was confirmed by clinical disease activity scores, histopathology, and serum amyloid A levels. RNA was isolated from full length sections of colon and expression of inflammatory genes was examined by real-time PCR array. Only statistically significant (p<0.05) genes that showed a > 3-fold difference in expression were analyzed. 16 genes were increased in the acute phase (day 5-24), whereas 0 genes were increased in the chronic phase (day 43-50). One gene was decreased in the acute phase, whereas 27 genes were decreased in the chronic phase. Six genes were increased throughout all phases (CSF-3, Cxcl1, Cxcl2, Cxcl5, S100a8 and TNFalpha). As expected, many acute phase cytokines (Th1 and Th17) and neutrophil chemokines were increased in the acute phase. IL1beta, IL6, IL17b and IL22 were increased in the acute phase but not the chronic phase. Interestingly, a substantial number of cytokines, chemokines and growth factors were decreased in the chronic phase. Similar changes were seen at the protein level for CSF-3, IL1b, IL6 and Ccl3 as measured by multiplex ELISA from colon tissue extracts. As expected, gene expression analysis was a more sensitive method with which to examine inflammatory cytokines in the colonic mucosa. Initial expression profiling studies suggests that innate inflammatory pathway genes are involved in the early phase of DSS induced colitis. Only a few of these genes maintained increased expression levels into the chronic phase, including TNFalpha. Increased expression of growth factors such as CSF-3 likely represents the healing seen in this model. It will be important to analyze these significant proteins in mesenteric lymph node derived T cells. Expression profiling facilitated the identification of inflammatory pathways involved in chronic colitis in a DSS mouse model and may help specify important target pathways in other animal models of IBD." @default.
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- W2330791323 date "2011-12-01" @default.
- W2330791323 modified "2023-10-05" @default.
- W2330791323 title "Expression profiling of inflammatory genes identifies differences in the acute and chronic phases of DSS induced colitis" @default.
- W2330791323 doi "https://doi.org/10.1097/00054725-201112002-00288" @default.
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