FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2330821640> ?p ?o ?g. }

• W2330821640 endingPage "2191" @default.
• W2330821640 startingPage "2181" @default.
• W2330821640 abstract "Insulin-like growth factor I (IGF-I) and its cognate receptor (IGF-1R) contribute to normal cell function and to tumorigenesis. The role of IGF-I signaling in tumor growth has been demonstrated in vivo using nucleic acid-based strategies. Here, we designed the first 10–23 DNAzymes directed against IGF-I mRNA. Unlike antisense approaches and RNA interference that require protein catalysis, DNAzymes catalyze protein-free RNA cleavage. We identified target sequences and measured catalytic properties of differently designed DNAzymes on short synthetic RNA targets and on in vitro transcribed IGF-I mRNA. The most efficient cleavers were then transfected into cells, and their inhibitory effect was analyzed using reporter gene assays. We found that increasing the size of DNAzyme flanking sequences and modifications of the termini with 2′-O-methyl residues improved cleavage rates of target RNAs. Modification of the catalytic loop with six 2′-O-methyl ribonucleotides at nonessential positions increased or decreased catalytic efficiency depending on the mRNA target site. In cells, DNAzymes with 2′-O-methyl-modified catalytic cores and flanking sequences were able to inhibit reporter gene activity because of specific recognition and cleavage of IGF-I mRNA sequences. Mutant DNAzymes with inactive catalytic cores were unable to block reporter gene expression, demonstrating that the RNA cleaving ability of 10–23 DNAzymes contributed to inhibitory mechanisms. Our results show that nuclease-resistant 2′-O-methyl-modified DNAzymes with high catalytic efficiencies are useful for inhibiting IGF-I gene function in cells." @default.
• W2330821640 created "2016-06-24" @default.
• W2330821640 creator A5004630053 @default.
• W2330821640 creator A5005575509 @default.
• W2330821640 creator A5008308460 @default.
• W2330821640 creator A5014734300 @default.
• W2330821640 creator A5071434696 @default.
• W2330821640 date "2012-03-08" @default.
• W2330821640 modified "2023-10-16" @default.
• W2330821640 title "Targeting Insulin-like Growth Factor I with 10–23 DNAzymes: 2′-O-Methyl Modifications in the Catalytic Core Enhance mRNA Cleavage" @default.
• W2330821640 cites W1485999252 @default.
• W2330821640 cites W1513052362 @default.
• W2330821640 cites W1542758544 @default.
• W2330821640 cites W1582486192 @default.
• W2330821640 cites W1634351 @default.
• W2330821640 cites W1653978584 @default.
• W2330821640 cites W1824972792 @default.
• W2330821640 cites W1942178888 @default.
• W2330821640 cites W1964607399 @default.
• W2330821640 cites W1972861367 @default.
• W2330821640 cites W1976579101 @default.
• W2330821640 cites W1977383342 @default.
• W2330821640 cites W1978922759 @default.
• W2330821640 cites W1987340677 @default.
• W2330821640 cites W1992696785 @default.
• W2330821640 cites W1993670509 @default.
• W2330821640 cites W1995442031 @default.
• W2330821640 cites W2000742881 @default.
• W2330821640 cites W2010759469 @default.
• W2330821640 cites W2011567260 @default.
• W2330821640 cites W2014075230 @default.
• W2330821640 cites W2014496171 @default.
• W2330821640 cites W2014970772 @default.
• W2330821640 cites W2015878656 @default.
• W2330821640 cites W2017073614 @default.
• W2330821640 cites W2025989537 @default.
• W2330821640 cites W2027614530 @default.
• W2330821640 cites W2030098441 @default.
• W2330821640 cites W2030431257 @default.
• W2330821640 cites W2031662022 @default.
• W2330821640 cites W2036471757 @default.
• W2330821640 cites W2041888883 @default.
• W2330821640 cites W2042865275 @default.
• W2330821640 cites W2043735713 @default.
• W2330821640 cites W2044006090 @default.
• W2330821640 cites W2045452812 @default.
• W2330821640 cites W2045653120 @default.
• W2330821640 cites W2052322311 @default.
• W2330821640 cites W2055760422 @default.
• W2330821640 cites W2058096411 @default.
• W2330821640 cites W2061045375 @default.
• W2330821640 cites W2063865555 @default.
• W2330821640 cites W2073096559 @default.
• W2330821640 cites W2076348849 @default.
• W2330821640 cites W2078446192 @default.
• W2330821640 cites W2086654802 @default.
• W2330821640 cites W2086783930 @default.
• W2330821640 cites W2094997688 @default.
• W2330821640 cites W2106991852 @default.
• W2330821640 cites W2111799987 @default.
• W2330821640 cites W2119303797 @default.
• W2330821640 cites W2129538373 @default.
• W2330821640 cites W2132697082 @default.
• W2330821640 cites W2139584857 @default.
• W2330821640 cites W2142467427 @default.
• W2330821640 cites W2142695285 @default.
• W2330821640 cites W2150167472 @default.
• W2330821640 cites W2153461732 @default.
• W2330821640 cites W2158043342 @default.
• W2330821640 cites W2170887875 @default.
• W2330821640 cites W2180627854 @default.
• W2330821640 cites W2391523750 @default.
• W2330821640 cites W4237468509 @default.
• W2330821640 cites W61404373 @default.
• W2330821640 cites W773751364 @default.
• W2330821640 doi "https://doi.org/10.1021/bi201532q" @default.
• W2330821640 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22352843" @default.
• W2330821640 hasPublicationYear "2012" @default.
• W2330821640 type Work @default.
• W2330821640 sameAs 2330821640 @default.
• W2330821640 citedByCount "41" @default.
• W2330821640 countsByYear W23308216402012 @default.
• W2330821640 countsByYear W23308216402013 @default.
• W2330821640 countsByYear W23308216402014 @default.
• W2330821640 countsByYear W23308216402015 @default.
• W2330821640 countsByYear W23308216402016 @default.
• W2330821640 countsByYear W23308216402017 @default.
• W2330821640 countsByYear W23308216402018 @default.
• W2330821640 countsByYear W23308216402019 @default.
• W2330821640 countsByYear W23308216402020 @default.
• W2330821640 countsByYear W23308216402021 @default.
• W2330821640 countsByYear W23308216402022 @default.
• W2330821640 countsByYear W23308216402023 @default.
• W2330821640 crossrefType "journal-article" @default.
• W2330821640 hasAuthorship W2330821640A5004630053 @default.
• W2330821640 hasAuthorship W2330821640A5005575509 @default.
• W2330821640 hasAuthorship W2330821640A5008308460 @default.
• W2330821640 hasAuthorship W2330821640A5014734300 @default.

• next