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- W2331096465 abstract "Polyacrylamide gel electrophoresis techniques performed in constant (CDGE) or gradient (DGGE) denaturing gels that are commonly used to detect DNA mutations were adapted for the detection of mutations directly in dsRNA molecules. The techniques were applied to investigate genomic differences between a highly pathogenic avian reovirus strain (S1133wt) and its cold-adapted non-pathogenic strain (P100) used as a vaccine, in an attempt to identify mutated virulence gene(s) that had led to viral attenuation. Wild and vaccine strains presented identical electrophoretic profiles of their 10-dsRNA genomic segments in standard SDS-PAGE. Under denaturing conditions, migration differences between the two strains were observed in many genes, including M1, M2 and S1, three genes associated with viral pathogenesis. The techniques are fast, simple to perform, and may prove valuable to screen for mutations and polymorphisms in other important dsRNA viruses, such as rotaviruses, birnaviruses and several plant viruses. DOI: http://dx.doi.org/10.17525/vrrjournal.v12i1-2.12" @default.
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- W2331096465 date "2007-09-15" @default.
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- W2331096465 title "DETECTION OF MUTATIONS IN AVIAN REOVIRUS dsRNA GENOME BY DENATURING GRADIENT (DGGE) AND CONSTANT DENATURING (CDGE) GEL ELECTROPHORESIS" @default.
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- W2331096465 doi "https://doi.org/10.17525/vrrjournal.v12i1-2.12" @default.
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