FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2331213893> ?p ?o ?g. }

• W2331213893 endingPage "836" @default.
• W2331213893 startingPage "824" @default.
• W2331213893 abstract "Pancreatic gene transfer could be useful to treat several diseases, such as diabetes mellitus, cystic fibrosis, chronic pancreatitis, or pancreatic cancer. Helper-dependent adenoviral vectors (HDAds) are promising tools for gene therapy because of their large cloning capacity, high levels of transgene expression, and long-term persistence in immunocompetent animals. Nevertheless, the ability of HDAds to transduce the pancreas in vivo has not been investigated yet. Here, we have generated HDAds carrying pancreas-specific expression cassettes, that is, driven either by the elastase or insulin promoter, using a novel and convenient plasmid family and homologous recombination in bacteria. These HDAds were delivered to the pancreas of immunocompetent mice via intrapancreatic duct injection. HDAds, encoding a CMV-GFP reporter cassette, were able to transduce acinar and islet cells, but transgene expression was lost 15 days postinjection in correlation with severe lymphocytic infiltration. When HDAds encoding GFP under the control of the specific elastase promoter were used, expression was detected in acinar cells, but similarly, the expression almost disappeared 30 days postinjection and lymphocytic infiltration was also observed. In contrast, long-term transgene expression (>8 months) was achieved with HDAds carrying the insulin promoter and the secretable alkaline phosphatase as the reporter gene. Notably, transduction of the liver, the preferred target for adenovirus, was minimal by this route of delivery. These data indicate that HDAds could be used for pancreatic gene therapy but that selection of the expression cassette is of critical importance to achieve long-term expression of the transgene in this tissue." @default.
• W2331213893 created "2016-06-24" @default.
• W2331213893 creator A5017417129 @default.
• W2331213893 creator A5023088425 @default.
• W2331213893 creator A5026416449 @default.
• W2331213893 creator A5034413746 @default.
• W2331213893 creator A5040007812 @default.
• W2331213893 creator A5044058894 @default.
• W2331213893 creator A5061503278 @default.
• W2331213893 creator A5074346990 @default.
• W2331213893 creator A5078994554 @default.
• W2331213893 creator A5085641483 @default.
• W2331213893 creator A5087953145 @default.
• W2331213893 date "2014-09-01" @default.
• W2331213893 modified "2023-10-18" @default.
• W2331213893 title "Pancreatic Transduction by Helper-Dependent Adenoviral Vectors via Intraductal Delivery" @default.
• W2331213893 cites W1538810590 @default.
• W2331213893 cites W1969210504 @default.
• W2331213893 cites W1980639998 @default.
• W2331213893 cites W1980890790 @default.
• W2331213893 cites W1982533894 @default.
• W2331213893 cites W1992033402 @default.
• W2331213893 cites W1992117647 @default.
• W2331213893 cites W1992912012 @default.
• W2331213893 cites W2003955696 @default.
• W2331213893 cites W2007276010 @default.
• W2331213893 cites W2013220929 @default.
• W2331213893 cites W2024189842 @default.
• W2331213893 cites W2026742469 @default.
• W2331213893 cites W2027717772 @default.
• W2331213893 cites W2030920503 @default.
• W2331213893 cites W2035590181 @default.
• W2331213893 cites W2035600956 @default.
• W2331213893 cites W2038576765 @default.
• W2331213893 cites W2043741727 @default.
• W2331213893 cites W2048324572 @default.
• W2331213893 cites W2054794825 @default.
• W2331213893 cites W2056738819 @default.
• W2331213893 cites W2056776686 @default.
• W2331213893 cites W2058315462 @default.
• W2331213893 cites W2060281994 @default.
• W2331213893 cites W2060965112 @default.
• W2331213893 cites W2067660751 @default.
• W2331213893 cites W2072266400 @default.
• W2331213893 cites W2073107777 @default.
• W2331213893 cites W2075921029 @default.
• W2331213893 cites W2078035839 @default.
• W2331213893 cites W2091081049 @default.
• W2331213893 cites W2095615635 @default.
• W2331213893 cites W2117902695 @default.
• W2331213893 cites W2118433531 @default.
• W2331213893 cites W2121672863 @default.
• W2331213893 cites W2126400402 @default.
• W2331213893 cites W2129754384 @default.
• W2331213893 cites W2132875472 @default.
• W2331213893 cites W2139880925 @default.
• W2331213893 cites W2141063928 @default.
• W2331213893 cites W2151226480 @default.
• W2331213893 cites W2152254495 @default.
• W2331213893 cites W2157885364 @default.
• W2331213893 cites W2174852485 @default.
• W2331213893 cites W2233696942 @default.
• W2331213893 cites W2323123215 @default.
• W2331213893 cites W2324521397 @default.
• W2331213893 doi "https://doi.org/10.1089/hum.2013.182" @default.
• W2331213893 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4175029" @default.
• W2331213893 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25046147" @default.
• W2331213893 hasPublicationYear "2014" @default.
• W2331213893 type Work @default.
• W2331213893 sameAs 2331213893 @default.
• W2331213893 citedByCount "8" @default.
• W2331213893 countsByYear W23312138932015 @default.
• W2331213893 countsByYear W23312138932016 @default.
• W2331213893 countsByYear W23312138932017 @default.
• W2331213893 countsByYear W23312138932019 @default.
• W2331213893 countsByYear W23312138932020 @default.
• W2331213893 countsByYear W23312138932022 @default.
• W2331213893 crossrefType "journal-article" @default.
• W2331213893 hasAuthorship W2331213893A5017417129 @default.
• W2331213893 hasAuthorship W2331213893A5023088425 @default.
• W2331213893 hasAuthorship W2331213893A5026416449 @default.
• W2331213893 hasAuthorship W2331213893A5034413746 @default.
• W2331213893 hasAuthorship W2331213893A5040007812 @default.
• W2331213893 hasAuthorship W2331213893A5044058894 @default.
• W2331213893 hasAuthorship W2331213893A5061503278 @default.
• W2331213893 hasAuthorship W2331213893A5074346990 @default.
• W2331213893 hasAuthorship W2331213893A5078994554 @default.
• W2331213893 hasAuthorship W2331213893A5085641483 @default.
• W2331213893 hasAuthorship W2331213893A5087953145 @default.
• W2331213893 hasBestOaLocation W23312138932 @default.
• W2331213893 hasConcept C102230213 @default.
• W2331213893 hasConcept C104317684 @default.
• W2331213893 hasConcept C111599444 @default.
• W2331213893 hasConcept C134018914 @default.
• W2331213893 hasConcept C135983454 @default.
• W2331213893 hasConcept C150194340 @default.
• W2331213893 hasConcept C15152581 @default.
• W2331213893 hasConcept C153911025 @default.

• next