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- W2331220004 abstract "Event Abstract Back to Event Comparing mechanical properties of silk-coated and collagen-coated hydroxyapatite scaffolds Diana Castillo1, Sergio Montelongo1, Joseph Pearson1, Joo L. Ong1 and Teja Guda1 1 University of Texas at San Antonio and University of Texas Health Science Center at San Antonio, Biomedical Engineering, United States Introduction: Considerable interest has been given to various fabrication techniques of scaffolds to seek different means of overcoming bone loss. Studies demonstrate that scaffolds act as temporary matrixes at the site of injury to promote cell proliferation, which in turn promote tissue regeneration[1]. Various coatings and architectural factors such as porosity are taken into account when enhancing the biomaterial. For example, the pore size of scaffolds should be about 100µm-300µm wide for the migration of cells and formation of bone and capillaries[2]. Collagen may be used to coat scaffolds, as it is the main protein component in bone and reveals exceptional biocompatibility[3]. Silk films are also used as coatings, as they improve cell attachment and bone formation[4]. This study focuses on constructing hydroxyapatite scaffolds with different coatings to identify samples with optimal mechanical properties to mimic physiological structures in order to lead to bone regeneration. Materials and Methods: Scaffolds were constructed from porous cylindrical polyurethane templates which were coated with a hydroxyapatite (HA) slurry and sintered. Two groups were formed: Group 1 consisted of collagen type I coated scaffolds of several concentrations (0.05%, 0.1%, and 0.25%) and Group 2 was comprised of silk coated scaffolds of varying concentrations (3%, 5%, and 7%). In addition to testing several concentrations, two submerging techniques were used: dipping the scaffold into the coating solution for a total of 6 times at 5 minute intervals, or soaking the scaffold into the solution for 30 minutes straight. Each sample was analyzed using a helium pycnometer in order to detect if scaffolds maintained their porosity after they were coated. Scanning Electron Microscopy was used to measure the trabecular thickness and pore size before and after coatings to identify if any pores were clogged with specific coating concentrations. Compression testing using the MTS Insight 5 was performed to compare the strength of silk and collagen coated scaffolds which were compared to a control group with no additional coating to the HA surface. Finally, One-way ANOVA and Tukey test were the statistical tests used (p<0.05) for data analysis. Results and Discussion: Scaffolds coated with 0.05% collagen for 30 minutes showed the strongest trend for an increase in toughness from the uncoated scaffolds (p=0.082) (Figure 1). Scaffolds coated with 7% silk for 30 minutes had an increase in ultimate stress (p= 0.001) and toughness (p<0.001) compared to its uncoated control group (Figure 2). It was identified that the trabecular thickness and pore size (~320µm) of each scaffold was not significantly affected after the coating of collagen or silk, indicating that they retained their open porous architecture after each coating (p>>0.05) (Figure 3). Conclusions: Lower concentrations of collagen may have resulted in a uniform thin film-like coating on the scaffolds in contrast to higher collagen concentrations which potentially led to local agglomerations with minimal impact on impeding fracture crack growth. The silk coatings were not as uniform as collagen coatings and there were slightly visible uneven films on the surface of the scaffold. It is not certain that the silk penetrated deep into the center of the scaffolds, but rather attached mostly to the surface, giving it lower mechanical properties than that of collagen coated HA scaffolds. References:[1] Dhandayuthapani, Brahatheeswaran, et al. Polymeric scaffolds in tissue engineering application: a review. International Journal of Polymer Science 2011 (2011).[2] Karageorgiou, Vassilis, and David Kaplan. Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials 26.27 (2005): 5474-5491.[3] Glowacki, Julie, and Shuichi Mizuno. Collagen scaffolds for tissue engineering. Biopolymers 89.5 (2008): 338-344.[4] Wang, Yongzhong, et al. Cartilage tissue engineering with silk scaffolds and human articular chondrocytes. Biomaterials 27.25 (2006): 4434-4442. Keywords: Bone Regeneration, biomaterial, Scaffold, mechanical property Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Mechanical properties of biomaterials Citation: Castillo D, Montelongo S, Pearson J, Ong JL and Guda T (2016). Comparing mechanical properties of silk-coated and collagen-coated hydroxyapatite scaffolds. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.00733 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Diana Castillo Sergio Montelongo Joseph Pearson Joo L Ong Teja Guda Google Diana Castillo Sergio Montelongo Joseph Pearson Joo L Ong Teja Guda Google Scholar Diana Castillo Sergio Montelongo Joseph Pearson Joo L Ong Teja Guda PubMed Diana Castillo Sergio Montelongo Joseph Pearson Joo L Ong Teja Guda Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page." @default.
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- W2331220004 title "Comparing mechanical properties of silk-coated and collagen-coated hydroxyapatite scaffolds" @default.
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