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• W2331246144 endingPage "4924" @default.
• W2331246144 startingPage "4917" @default.
• W2331246144 abstract "Fluorescence and Raman inner filter effects (IFE) cause spectral distortion and nonlinearity between spectral signal intensity with increasing analyte concentration. Convenient and effective correction of fluorescence IFE has been an active research goal for decades. Presented herein is the finding that fluorescence and Raman IFE can be reliably corrected using the equation I(corr)/I(obsd) = 10(dxAx + dmAm) when the effective excitation and emission path lengths, dx and dm, of a fluorophotometer are determined by simple linear curve-fitting of Raman intensities of a series of water Raman reference samples that have known degrees of Raman IFEs. The path lengths derived with one set of Raman measurements at one specific excitation wavelength are effective for correcting fluorescence and Raman IFEs induced by any chromophore or fluorophore, regardless of the excitation and emission wavelengths. The IFE-corrected fluorescence intensities are linearly correlated to fluorophore concentration over 5 orders of magnitude (from 5.9 nM to 0.59 mM) for 2-aminopurine in a 1 cm × 0.17 cm fluorescence cuvette. This water Raman-based method is easy to implement. It does not involve complicated instrument geometry determination or difficult data manipulation. This work should be of broad significance to physical and biological sciences given the popularity of fluorescence techniques in analytical applications." @default.
• W2331246144 created "2016-06-24" @default.
• W2331246144 creator A5026178052 @default.
• W2331246144 creator A5078350089 @default.
• W2331246144 creator A5079701813 @default.
• W2331246144 date "2015-04-22" @default.
• W2331246144 modified "2023-09-29" @default.
• W2331246144 title "Using Water Raman Intensities To Determine the Effective Excitation and Emission Path Lengths of Fluorophotometers for Correcting Fluorescence Inner Filter Effect" @default.
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• W2331246144 doi "https://doi.org/10.1021/acs.analchem.5b00513" @default.
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