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• W2331259851 startingPage "12382" @default.
• W2331259851 abstract "Enzyme activity assay is an important method in clinical diagnostics. However, conventional enzyme activity assay suffers from apparent interference from the sample matrix. Herein, we present a new format of enzyme activity assay that can effectively eliminate the effects of the sample matrix. The key is a 96-well microplate modified with molecularly imprinted polymer (MIP) prepared according to a newly proposed method called boronate affinity-based oriented surface imprinting. Alkaline phosphatase (ALP), a glycoprotein enzyme that has been routinely used as an indicator for several diseases in clinical tests, was taken as a representative target enzyme. The prepared MIP exhibited strong affinity toward the template enzyme (with a dissociation constant of 10–10 M) as well as superb tolerance for interference. Thus, the enzyme molecules in a complicated sample matrix could be specifically captured and cleaned up for enzyme activity assay, which eliminated the interference from the sample matrix. On the other hand, because the boronate affinity MIP could well retain the enzymatic activity of glycoprotein enzymes, the enzyme captured by the MIP was directly used for activity assay. Thus, additional assay time and possible enzyme or activity loss due to an enzyme release step required by other methods were avoided. Assay of ALP in human serum was successfully demonstrated, suggesting a promising prospect of the proposed method in real-world applications." @default.
• W2331259851 created "2016-06-24" @default.
• W2331259851 creator A5050544425 @default.
• W2331259851 creator A5058479146 @default.
• W2331259851 date "2014-12-02" @default.
• W2331259851 modified "2023-10-07" @default.
• W2331259851 title "Enzyme Activity Assay of Glycoprotein Enzymes Based on a Boronate Affinity Molecularly Imprinted 96-Well Microplate" @default.
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• W2331259851 doi "https://doi.org/10.1021/ac503778w" @default.
• W2331259851 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25405946" @default.
• W2331259851 hasPublicationYear "2014" @default.
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