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• W2331323254 abstract "Bottom up assembly of functional molecular ensembles with novel properties emerging from composition and arrangement of its constituents is a prime goal of nanotechnology. By single-molecule cut-and-paste we assembled binding sites for malachite green in a molecule-by-molecule assembly process from the two halves of a split aptamer. We show that only a perfectly joined binding site immobilizes the fluorophore and enhances the fluorescence quantum yield by several orders of magnitude. To corroborate the robustness of this approach we produced a micrometer-sized structure consisting of more than 500 reconstituted binding sites. To the best of our knowledge, this is the first demonstration of one by one bottom up functional biomolecular assembly." @default.
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• W2331323254 date "2012-04-06" @default.
• W2331323254 modified "2023-09-26" @default.
• W2331323254 title "Functional Assembly of Aptamer Binding Sites by Single-Molecule Cut-and-Paste" @default.
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• W2331323254 doi "https://doi.org/10.1021/nl300422y" @default.
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