FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2331367600> ?p ?o ?g. }

• W2331367600 endingPage "3160" @default.
• W2331367600 startingPage "3153" @default.
• W2331367600 abstract "Rapid analysis of pathogenic bacteria is essential for food and water control to preserve the public health. Therefore, we report on a chemiluminescence (CL) flow-through DNA microarray assay for the rapid and sensitive quantification of the pathogenic bacteria Escherichia coli O157:H7, Salmonella enterica, and Campylobacter jejuni in water. Using the stopped polymerase chain reaction (PCR) strategy, the amount of amplified target DNA was strongly dependent on the applied cell concentration. The amplification was stopped at the logarithmic phase of the PCR to quantify the DNA products on the DNA microarray chip. The generation of single-stranded DNA sequences is essential for DNA hybridization assays on microarrays. Therefore, the DNA strands of the PCR products were separated by streptavidin-conjugated magnetic nanoparticles. This was achieved by introducing a reverse primer labeled with biotin together with a digoxigenin labeled forward primer for CL microarray imaging. A conjugate of an antidigoxigenin antibody and horseradish peroxidase recognized the digoxigenin-labeled antistrands bound to the probes on the microarray surface and catalyzed the reaction of luminol and hydrogen peroxide. The generated light emission was recorded by a sensitive charge-coupled device (CCD) camera. The quantification was conducted by a flow-through CL microarray readout system. The DNA microarrays were based on an NHS-activated poly(ethylene glycol)-modified glass substrate. The DNA probes which have the same DNA sequence as the reverse primer were immobilized on this surface. The full assay was characterized by spiking experiments with heat-inactivated bacteria in water. The total assay time was 3.5 h, and the detection limits determined on CL microarrays were for E. coli O157:H7, S. enterica, and C. jejuni 136, 500, and 1 cell/mL, respectively. The results of the DNA microarray assay were comparable to the SYBR green-based assays analyzed with a real-time PCR device. The advantage of the new microarray analysis method is seen in the ability of a high multiplex degree on DNA microarrays, the high specificity of DNA hybridization on DNA microarrays, and the possibility to get quantitative results on an automated CL flow-through microarray analysis system." @default.
• W2331367600 created "2016-06-24" @default.
• W2331367600 creator A5013814528 @default.
• W2331367600 creator A5015095882 @default.
• W2331367600 creator A5080674213 @default.
• W2331367600 date "2011-03-21" @default.
• W2331367600 modified "2023-10-03" @default.
• W2331367600 title "Sensitive Quantification of Escherichia coli O157:H7, Salmonella enterica, and Campylobacter jejuni by Combining Stopped Polymerase Chain Reaction with Chemiluminescence Flow-Through DNA Microarray Analysis" @default.
• W2331367600 cites W1969096019 @default.
• W2331367600 cites W1977569346 @default.
• W2331367600 cites W1978120111 @default.
• W2331367600 cites W1979275379 @default.
• W2331367600 cites W1984869695 @default.
• W2331367600 cites W1994283816 @default.
• W2331367600 cites W2016944275 @default.
• W2331367600 cites W2026055882 @default.
• W2331367600 cites W2031515697 @default.
• W2331367600 cites W2037908308 @default.
• W2331367600 cites W2040024947 @default.
• W2331367600 cites W2053439080 @default.
• W2331367600 cites W2053582098 @default.
• W2331367600 cites W2058420239 @default.
• W2331367600 cites W2062004651 @default.
• W2331367600 cites W2066714928 @default.
• W2331367600 cites W2079721781 @default.
• W2331367600 cites W2089689338 @default.
• W2331367600 cites W2093310108 @default.
• W2331367600 cites W2102616257 @default.
• W2331367600 cites W2122249846 @default.
• W2331367600 cites W2138672286 @default.
• W2331367600 cites W2157098397 @default.
• W2331367600 cites W2157706441 @default.
• W2331367600 cites W2157888044 @default.
• W2331367600 cites W2160815528 @default.
• W2331367600 cites W4211027422 @default.
• W2331367600 cites W4211041611 @default.
• W2331367600 doi "https://doi.org/10.1021/ac2002214" @default.
• W2331367600 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21417213" @default.
• W2331367600 hasPublicationYear "2011" @default.
• W2331367600 type Work @default.
• W2331367600 sameAs 2331367600 @default.
• W2331367600 citedByCount "89" @default.
• W2331367600 countsByYear W23313676002012 @default.
• W2331367600 countsByYear W23313676002013 @default.
• W2331367600 countsByYear W23313676002014 @default.
• W2331367600 countsByYear W23313676002015 @default.
• W2331367600 countsByYear W23313676002016 @default.
• W2331367600 countsByYear W23313676002017 @default.
• W2331367600 countsByYear W23313676002018 @default.
• W2331367600 countsByYear W23313676002019 @default.
• W2331367600 countsByYear W23313676002020 @default.
• W2331367600 countsByYear W23313676002021 @default.
• W2331367600 countsByYear W23313676002022 @default.
• W2331367600 countsByYear W23313676002023 @default.
• W2331367600 crossrefType "journal-article" @default.
• W2331367600 hasAuthorship W2331367600A5013814528 @default.
• W2331367600 hasAuthorship W2331367600A5015095882 @default.
• W2331367600 hasAuthorship W2331367600A5080674213 @default.
• W2331367600 hasConcept C104317684 @default.
• W2331367600 hasConcept C150194340 @default.
• W2331367600 hasConcept C153911025 @default.
• W2331367600 hasConcept C178790620 @default.
• W2331367600 hasConcept C185592680 @default.
• W2331367600 hasConcept C2776957140 @default.
• W2331367600 hasConcept C2777563447 @default.
• W2331367600 hasConcept C2779607309 @default.
• W2331367600 hasConcept C2779910956 @default.
• W2331367600 hasConcept C49105822 @default.
• W2331367600 hasConcept C523546767 @default.
• W2331367600 hasConcept C54355233 @default.
• W2331367600 hasConcept C547475151 @default.
• W2331367600 hasConcept C552990157 @default.
• W2331367600 hasConcept C55493867 @default.
• W2331367600 hasConcept C86803240 @default.
• W2331367600 hasConcept C95371953 @default.
• W2331367600 hasConceptScore W2331367600C104317684 @default.
• W2331367600 hasConceptScore W2331367600C150194340 @default.
• W2331367600 hasConceptScore W2331367600C153911025 @default.
• W2331367600 hasConceptScore W2331367600C178790620 @default.
• W2331367600 hasConceptScore W2331367600C185592680 @default.
• W2331367600 hasConceptScore W2331367600C2776957140 @default.
• W2331367600 hasConceptScore W2331367600C2777563447 @default.
• W2331367600 hasConceptScore W2331367600C2779607309 @default.
• W2331367600 hasConceptScore W2331367600C2779910956 @default.
• W2331367600 hasConceptScore W2331367600C49105822 @default.
• W2331367600 hasConceptScore W2331367600C523546767 @default.
• W2331367600 hasConceptScore W2331367600C54355233 @default.
• W2331367600 hasConceptScore W2331367600C547475151 @default.
• W2331367600 hasConceptScore W2331367600C552990157 @default.
• W2331367600 hasConceptScore W2331367600C55493867 @default.
• W2331367600 hasConceptScore W2331367600C86803240 @default.
• W2331367600 hasConceptScore W2331367600C95371953 @default.
• W2331367600 hasIssue "8" @default.
• W2331367600 hasLocation W23313676001 @default.
• W2331367600 hasLocation W23313676002 @default.
• W2331367600 hasOpenAccess W2331367600 @default.
• W2331367600 hasPrimaryLocation W23313676001 @default.
• W2331367600 hasRelatedWork W1582851669 @default.

• next