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- W2331387266 abstract "In Australia DNA ploidy is emerging as a clinically useful investigation for ongoing management of malignant disorders, We compare the results of DNA ploidy analysis performed by video image analysis(VIA) and flow cytometry(FC) in two independent laboratorles.Seventy-eight cases of primary breast carcinoma were retrieved from the files of CGMC.The tissues were formalin-fixed paraffin- embedded.The tissue for flow cytometry was prepared by a modified Hedley technique and for image analysis the sections were stained with Feulgen stain.Measurement of DNA ploidy was performed on a Coulter Epics Profile II flow cytometer and a Leading Edge MD20 video image analysis system.Of the 78 cases 5 were uninterpretable by flow cytometry and 13 by image analysis. In 11 cases the ploidy analysis varied between the two techniques with 7 of the 11 divergent assessments having outcome implications.Of the 78 cases tested 39 were DNA diploid by FC (38 by VIA with 3 hyperdlplold), 14 aneuploid(l 1 by VIA), and 20 tetraploid(l3 by VIA). The reasons for the discrepancies between the results obtained by the two techniques were 1 the quality of the fixation;2 the use of archival material for flow cytometry;3.the difficulty In coming to terms with differing definitions and 4, the low cell yields achieved in some cases by both techniques.We believe that further assessment of data is required but that any ploidy result used in clinical practice should be a combination of VIA and FC analysis of each case." @default.
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- W2331387266 date "1993-01-01" @default.
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- W2331387266 title "A Comparative study between flow cytometry and video image analysis of DNA ploidy status in breast cancer" @default.
- W2331387266 doi "https://doi.org/10.1016/s0031-3025(16)35771-3" @default.
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