FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2331601844> ?p ?o ?g. }

• W2331601844 endingPage "3571" @default.
• W2331601844 startingPage "3563" @default.
• W2331601844 abstract "ABSTRACT Both prokaryotic and eukaryotic organisms possess mechanisms for the detoxification of heavy metals, and these mechanisms are found among distantly related species. We investigated the role of intracellular glutathione (GSH), which, in a large number of taxa, plays a role in protection against the toxicity of common heavy metals. Anaerobically grown Lactococcus lactis containing an inducible GSH synthesis pathway was used as a model organism. Its physiological condition allowed study of putative GSH-dependent uranyl detoxification mechanisms without interference from additional reactive oxygen species. By microcalorimetric measurements of metabolic heat during cultivation, it was shown that intracellular GSH attenuates the toxicity of uranium at a concentration in the range of 10 to 150 μM. In this concentration range, no effect was observed with copper, which was used as a reference for redox metal toxicity. At higher copper concentrations, GSH aggravated metal toxicity. Isothermal titration calorimetry revealed the endothermic binding of U(VI) to the carboxyl group(s) of GSH rather than to the reducing thiol group involved in copper interactions. The data indicate that the primary detoxifying mechanism is the intracellular sequestration of carboxyl-coordinated U(VI) into an insoluble complex with GSH. The opposite effects on uranyl and on copper toxicity can be related to the difference in coordination chemistry of the respective metal-GSH complexes, which cause distinct growth phase-specific effects on enzyme-metal interactions. IMPORTANCE Understanding microbial metal resistance is of particular importance for bioremediation, where microorganisms are employed for the removal of heavy metals from the environment. This strategy is increasingly being considered for uranium. However, little is known about the molecular mechanisms of uranyl detoxification. Existing studies of different taxa show little systematics but hint at a role of glutathione (GSH). Previous work could not unequivocally demonstrate a GSH function in decreasing the presumed uranyl-induced oxidative stress, nor could a redox-independent detoxifying action of GSH be identified. Combining metabolic calorimetry with cell number-based assays and genetics analysis enables a novel and general approach to quantify toxicity and relate it to molecular mechanisms. The results show that GSH-expressing microorganisms appear advantageous for uranyl bioremediation." @default.
• W2331601844 created "2016-06-24" @default.
• W2331601844 creator A5051546849 @default.
• W2331601844 creator A5064565374 @default.
• W2331601844 creator A5073996154 @default.
• W2331601844 creator A5079074480 @default.
• W2331601844 date "2016-06-15" @default.
• W2331601844 modified "2023-09-27" @default.
• W2331601844 title "Mechanism of Attenuation of Uranyl Toxicity by Glutathione in Lactococcus lactis" @default.
• W2331601844 cites W1531324256 @default.
• W2331601844 cites W1547421433 @default.
• W2331601844 cites W1547894132 @default.
• W2331601844 cites W1558751029 @default.
• W2331601844 cites W1602373035 @default.
• W2331601844 cites W1963771566 @default.
• W2331601844 cites W1964010174 @default.
• W2331601844 cites W1964610622 @default.
• W2331601844 cites W1965793981 @default.
• W2331601844 cites W1969993194 @default.
• W2331601844 cites W1971603036 @default.
• W2331601844 cites W1974572914 @default.
• W2331601844 cites W1982499931 @default.
• W2331601844 cites W1995732138 @default.
• W2331601844 cites W2003898032 @default.
• W2331601844 cites W2007854440 @default.
• W2331601844 cites W2026824450 @default.
• W2331601844 cites W2027732708 @default.
• W2331601844 cites W2035376533 @default.
• W2331601844 cites W2050711776 @default.
• W2331601844 cites W2065475071 @default.
• W2331601844 cites W2070884182 @default.
• W2331601844 cites W2079719786 @default.
• W2331601844 cites W2082259473 @default.
• W2331601844 cites W2086813956 @default.
• W2331601844 cites W2089457089 @default.
• W2331601844 cites W2090874848 @default.
• W2331601844 cites W2096046927 @default.
• W2331601844 cites W2096063115 @default.
• W2331601844 cites W2097951502 @default.
• W2331601844 cites W2107725151 @default.
• W2331601844 cites W2117615022 @default.
• W2331601844 cites W2135141843 @default.
• W2331601844 cites W2136428925 @default.
• W2331601844 cites W2152063968 @default.
• W2331601844 cites W2157660908 @default.
• W2331601844 cites W2160662164 @default.
• W2331601844 cites W2165852502 @default.
• W2331601844 cites W2170206509 @default.
• W2331601844 cites W24256299 @default.
• W2331601844 cites W2472485621 @default.
• W2331601844 cites W278709392 @default.
• W2331601844 doi "https://doi.org/10.1128/aem.00538-16" @default.
• W2331601844 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4959180" @default.
• W2331601844 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27060118" @default.
• W2331601844 hasPublicationYear "2016" @default.
• W2331601844 type Work @default.
• W2331601844 sameAs 2331601844 @default.
• W2331601844 citedByCount "13" @default.
• W2331601844 countsByYear W23316018442017 @default.
• W2331601844 countsByYear W23316018442018 @default.
• W2331601844 countsByYear W23316018442020 @default.
• W2331601844 countsByYear W23316018442021 @default.
• W2331601844 countsByYear W23316018442022 @default.
• W2331601844 countsByYear W23316018442023 @default.
• W2331601844 crossrefType "journal-article" @default.
• W2331601844 hasAuthorship W2331601844A5051546849 @default.
• W2331601844 hasAuthorship W2331601844A5064565374 @default.
• W2331601844 hasAuthorship W2331601844A5073996154 @default.
• W2331601844 hasAuthorship W2331601844A5079074480 @default.
• W2331601844 hasBestOaLocation W23316018441 @default.
• W2331601844 hasConcept C156911925 @default.
• W2331601844 hasConcept C178790620 @default.
• W2331601844 hasConcept C181199279 @default.
• W2331601844 hasConcept C185592680 @default.
• W2331601844 hasConcept C2775920511 @default.
• W2331601844 hasConcept C2778302132 @default.
• W2331601844 hasConcept C2778404011 @default.
• W2331601844 hasConcept C2780011460 @default.
• W2331601844 hasConcept C29730261 @default.
• W2331601844 hasConcept C523546767 @default.
• W2331601844 hasConcept C538909803 @default.
• W2331601844 hasConcept C54355233 @default.
• W2331601844 hasConcept C55493867 @default.
• W2331601844 hasConcept C79879829 @default.
• W2331601844 hasConcept C86803240 @default.
• W2331601844 hasConceptScore W2331601844C156911925 @default.
• W2331601844 hasConceptScore W2331601844C178790620 @default.
• W2331601844 hasConceptScore W2331601844C181199279 @default.
• W2331601844 hasConceptScore W2331601844C185592680 @default.
• W2331601844 hasConceptScore W2331601844C2775920511 @default.
• W2331601844 hasConceptScore W2331601844C2778302132 @default.
• W2331601844 hasConceptScore W2331601844C2778404011 @default.
• W2331601844 hasConceptScore W2331601844C2780011460 @default.
• W2331601844 hasConceptScore W2331601844C29730261 @default.
• W2331601844 hasConceptScore W2331601844C523546767 @default.
• W2331601844 hasConceptScore W2331601844C538909803 @default.
• W2331601844 hasConceptScore W2331601844C54355233 @default.
• W2331601844 hasConceptScore W2331601844C55493867 @default.

• next