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- W2331617231 abstract "Substances separated by chromatographic procedures from rapeseed frying oil that had been used for 66 days promoted the hemolysis of rabbit erythrocytes. The frying oil was applied to a column of silicic acid and eluted with isopropyl ether-hexane mixture, isopropyl ether, isopropyl ether-ether mixture, ether and acetone in turn. The ether fraction showed a hemolytic activity (C50; 50% hemolytic activity for rabbit erythrocyte) of 1.30mg. This active fraction was separated further by gel-permeation chromatography on a column of Toyopearl TSK-GEL HW-60 with chloroform-methanol mixture as the eluent. Two peaks appeared, of which the first contained glyceride dimer (GD) and second contained free fatty acid (FFA). C50 of the first fraction was 1.70mg and C50 of the second fraction was approximately 0.1mg, which was 44 times higher than that of the frying oil. GD and FFA appear to be significant hemolysis-promoting factors in frying oils, because in frying oils used for from 3 to 66 days, the contents of GD and FFA increased from 1 to 3% and from 20 to 200mg%, respectively, while the C50 of the oils decreased." @default.
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- W2331617231 date "1984-01-01" @default.
- W2331617231 modified "2023-09-26" @default.
- W2331617231 title "Separation of Rabbit Erythrocyte Hemolysis-promoting Substances in Frying Oil" @default.
- W2331617231 doi "https://doi.org/10.3358/shokueishi.25.494" @default.
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