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• W2331736282 abstract "1. To elucidate which tissues play a role as amino acids source for egg white protein synthesis in the steady state, 17.5 μCi of L-lysine-U-14C was injected intravenously into hens at the predicted ovulation time. Single Comb White Leghorn hens aged 60-70 weeks with 100% egg production rate were supplied a diet containing 0.71% of lysine. They were housed in a metabolic cage to monitor respiratory carbon dioxide for 5 or 10 hrs. At 5, 29 and 53 hrs after injection (29 and 53 hrs meant 5 hrs after predicted ovulation time on the second and third day after injection), three hens each were killed. Radioactivity in eggs, excreta, liver, oviduct and other tissues were determined.2. The recovery of 14C in respiratory carbon dioxide for 10 hrs was 8.70% and those in excreta were 2.39, 2.49 and 2.88% of dose at 5, 29 and 53 hrs after injection.3. The recoveries of 14C in the liver were 7. 15, 5.85 and 4.35% of dose at 5, 29 and 53 hrs after injection. The specific activities in duodenum, jejuno-ileum and pancreas at 5 hrs after injection, but the sizes of them were so small as a source of amino acids for egg white protein.4. The recovery of 14C in the plasma was 14.30% of dose at 5 hrs and decreased to 5.50% at 29 hrs and 4.26% of dose at 53 hrs after injection. That in the nonprotein fraction of plasma was 0.14% at 5 hrs after injection.5. The recoveries of 14C in yolks and follicles were 3.36, 11.89 and 14.38% of dose at 5, 29 and 53 hrs after injection, respectively. Eight yolks and follicles incorporated 14C at 5 hrs after injection. Second to fifth follicles incorporated almost the same amount of 14C at each period.6. The recoveries of 14C in the first, second and third egg whites were 5.08-7.13, 8.98-9.88 and 2.04% of dose, respectively. Those in the magnum were 14.59, 5.34 and 4.35% of dose, respectively. The sums of recovery of 14C in the egg white and in the magnum were 19.41-23.40% 0f dose. The magnum proteins with high radioactivity were synthesized during the radioactivity in the plasma was high. Then a portion, not all of synthesized protein in the magnum was transported into the first egg." @default.
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• W2331736282 date "1984-01-01" @default.
• W2331736282 modified "2023-10-14" @default.
• W2331736282 title "Intravenous lysine-14C incorporation into egg and tissue proteins of laying hens." @default.
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• W2331736282 doi "https://doi.org/10.2141/jpsa.21.57" @default.
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