Matches in SemOpenAlex for { <https://semopenalex.org/work/W2331740105> ?p ?o ?g. }
- W2331740105 endingPage "481" @default.
- W2331740105 startingPage "472" @default.
- W2331740105 abstract "Transcellular Ca(2+)transport in the late distal convoluted tubule and connecting tubule (DCT2/CNT) of the kidney is a finely controlled process mediated by the transient receptor potential vanilloid type 5 (TRPV5) channel. A complex-type-N-glycan bound at the extracellular residue Asn358 of TRPV5 through post-translational glycosylation has been postulated to regulate the activity of TRPV5 channels. Using in vitro Ca(2+)transport assays, immunoblot analysis, immunohistochemistry, patch clamp electrophysiology and total internal reflection fluorescence microscopy, it is demonstrated that the glycosidase β-galactosidase (β-gal), an enzyme that hydrolyzes galactose, stimulates TRPV5 channel activity. However, the activity of the non-glycosylated TRPV(N358Q)mutant was not altered in the presence of β-gal, showing that the stimulation is dependent on the presence of the TRPV5N-glycan. In addition, β-gal was found to stimulate transcellular Ca(2+)transport in isolated mouse primary DCT2/CNT cells. β-gal expression was detected in the apical membrane of the proximal tubules, and the protein was found in mouse urine. In summary, β-gal is present in the pro-urine from where it is thought to stimulate TRPV5 activity." @default.
- W2331740105 created "2016-06-24" @default.
- W2331740105 creator A5007406975 @default.
- W2331740105 creator A5009643907 @default.
- W2331740105 creator A5020688805 @default.
- W2331740105 creator A5031181174 @default.
- W2331740105 creator A5032275913 @default.
- W2331740105 creator A5054888289 @default.
- W2331740105 creator A5084777052 @default.
- W2331740105 date "2016-01-07" @default.
- W2331740105 modified "2023-09-27" @default.
- W2331740105 title "Urinary β-galactosidase stimulates Ca<sup>2+</sup>transport by stabilizing TRPV5 at the plasma membrane" @default.
- W2331740105 cites W1938250799 @default.
- W2331740105 cites W1969925688 @default.
- W2331740105 cites W1970825974 @default.
- W2331740105 cites W1979798409 @default.
- W2331740105 cites W1982442386 @default.
- W2331740105 cites W1994282992 @default.
- W2331740105 cites W1994755803 @default.
- W2331740105 cites W1998369937 @default.
- W2331740105 cites W2004125187 @default.
- W2331740105 cites W2012085559 @default.
- W2331740105 cites W2034759328 @default.
- W2331740105 cites W2037906340 @default.
- W2331740105 cites W2042304168 @default.
- W2331740105 cites W2042832021 @default.
- W2331740105 cites W2044327338 @default.
- W2331740105 cites W2045383172 @default.
- W2331740105 cites W2050565170 @default.
- W2331740105 cites W2051167270 @default.
- W2331740105 cites W2063834336 @default.
- W2331740105 cites W2074910307 @default.
- W2331740105 cites W2076936959 @default.
- W2331740105 cites W2080133117 @default.
- W2331740105 cites W2083748277 @default.
- W2331740105 cites W2118813807 @default.
- W2331740105 cites W2119240136 @default.
- W2331740105 cites W2120038848 @default.
- W2331740105 cites W2122037409 @default.
- W2331740105 cites W2126099817 @default.
- W2331740105 cites W2128688458 @default.
- W2331740105 cites W2129051779 @default.
- W2331740105 cites W2132537604 @default.
- W2331740105 cites W2135898296 @default.
- W2331740105 cites W2136041234 @default.
- W2331740105 cites W2144562803 @default.
- W2331740105 cites W2147011805 @default.
- W2331740105 cites W2159387890 @default.
- W2331740105 cites W2165627805 @default.
- W2331740105 cites W2167455527 @default.
- W2331740105 cites W4244273098 @default.
- W2331740105 doi "https://doi.org/10.1093/glycob/cwv172" @default.
- W2331740105 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26747426" @default.
- W2331740105 hasPublicationYear "2016" @default.
- W2331740105 type Work @default.
- W2331740105 sameAs 2331740105 @default.
- W2331740105 citedByCount "6" @default.
- W2331740105 countsByYear W23317401052017 @default.
- W2331740105 countsByYear W23317401052020 @default.
- W2331740105 countsByYear W23317401052021 @default.
- W2331740105 countsByYear W23317401052022 @default.
- W2331740105 countsByYear W23317401052023 @default.
- W2331740105 crossrefType "journal-article" @default.
- W2331740105 hasAuthorship W2331740105A5007406975 @default.
- W2331740105 hasAuthorship W2331740105A5009643907 @default.
- W2331740105 hasAuthorship W2331740105A5020688805 @default.
- W2331740105 hasAuthorship W2331740105A5031181174 @default.
- W2331740105 hasAuthorship W2331740105A5032275913 @default.
- W2331740105 hasAuthorship W2331740105A5054888289 @default.
- W2331740105 hasAuthorship W2331740105A5084777052 @default.
- W2331740105 hasBestOaLocation W23317401051 @default.
- W2331740105 hasConcept C120882062 @default.
- W2331740105 hasConcept C12554922 @default.
- W2331740105 hasConcept C155164980 @default.
- W2331740105 hasConcept C170493617 @default.
- W2331740105 hasConcept C176285220 @default.
- W2331740105 hasConcept C185592680 @default.
- W2331740105 hasConcept C28406088 @default.
- W2331740105 hasConcept C3018436504 @default.
- W2331740105 hasConcept C41625074 @default.
- W2331740105 hasConcept C55493867 @default.
- W2331740105 hasConcept C60903277 @default.
- W2331740105 hasConcept C86803240 @default.
- W2331740105 hasConcept C95444343 @default.
- W2331740105 hasConceptScore W2331740105C120882062 @default.
- W2331740105 hasConceptScore W2331740105C12554922 @default.
- W2331740105 hasConceptScore W2331740105C155164980 @default.
- W2331740105 hasConceptScore W2331740105C170493617 @default.
- W2331740105 hasConceptScore W2331740105C176285220 @default.
- W2331740105 hasConceptScore W2331740105C185592680 @default.
- W2331740105 hasConceptScore W2331740105C28406088 @default.
- W2331740105 hasConceptScore W2331740105C3018436504 @default.
- W2331740105 hasConceptScore W2331740105C41625074 @default.
- W2331740105 hasConceptScore W2331740105C55493867 @default.
- W2331740105 hasConceptScore W2331740105C60903277 @default.
- W2331740105 hasConceptScore W2331740105C86803240 @default.
- W2331740105 hasConceptScore W2331740105C95444343 @default.
- W2331740105 hasIssue "5" @default.