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• W2331862778 abstract "Event Abstract Back to Event Growth factor releasing micro strands promoted in vitro angiogenesis Afra Hadjizadeh1 and Houman Savoji2, 3 1 Amirkabir University of Technology, Department of Biomedical Engineering and 2Center of Excellence on Biomaterials, Iran 2 École Polytechnique de Montréal, CREPEC, Department of Chemical Engineering, Canada 3 École Polytechnique de Montréal, Institute of Biomedical Engineering, Canada Introduction: Growth factors (GFs) offer the potential to regulate tissue formation, maintenance or regeneration. These biomolecules as powerful signalling tools control cellular behavior, such as adhesion, proliferation, migration and differentiation.GFs are naturally occurring substances secreted by cells; to transfer signals between the cells and their micro environment, by binding and forming stable complexes to their receptors present on the cell surface. Since these molecules mostly are proteins, it is important to retain their biological activity upon inclusion of GFs into these materials and their optimized accessibility to obtain an efficient cell response. By inspiration from the nature, it is possible to incorporate extracellular matrix (ECM) to confine GFs mobility and control their temporal and special delivery. To this aim, different strategies have been used, including chemical or physical surface immobilization. Heparin sulfate; a sulfated, linear, unbranched polysaccharide, structurally composed of disaccharide repeated units, is present in the ECM. This macromolecule has many carboxyl groups which can be served for covalent reaction with surface amine groups of biomaterials[1]. In considering the above discussion, in the present study, it was decided to covalently graft heparin solfate onto the amin functionalized surface and then load VEGF into heparin chains to promote angiogenesis in vitro tissue culture system. Materials and Methods: The detailed fabrication and surface characterization of amin functionlized fibers have been previously reported[2]. Here, the immobilization of heparin was performed with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS onto the surface of 100-μm diameter poly (ethylene terephthalate) (PET) monofilaments, via an n-hepthylamine plasma polymer (HApp) bearing amine groups. The successful grafting onto the surface was confirmed by X- ray photoelecron spectroscopy analysis. Then VEGF were physically immobilized onto heparinized polymer fibers, as illustrated in Figure 1. During the entire process, fibers were maintained parallel in a Teflon holder ring (Figure 2 A). HUVECs were then seeded and grown on fibers for confluence. The holder was then embedded in a fibrin gel and culture medium added and the samples were incubated for 20 days. Cells on the fibers were stained for actin filaments, using TRITC–phalloidin, and counterstained with Sytoxgreen to highlight the position of the nuclei subsequently. Results and Discussion: The success grafting of the heparin onto the surface was confirmed by X- ray photoelecron spectroscopy analysis, showing the appearance of the sulfur element of about 4 % comparing to uncoated sample for which the sulfur was 0%. VEGF release was evaluated indirectly by cell culture experiment. As shown in figure 2 and 3, tube like structure formation occurred on the HUVECs seeded fibers embedded in the fibrin gel 3D system. Cell strands formed along the fibers and then branches were occurred from those strands forming vascular network like structures (figure 2B and C). The vessel formation on the VEGF loaded sample was higher than others as counted after 20 days of incubation (figure 3). Heparin mediated physically loaded VEGF onto the scaffolding biomaterials can release and promote angiogenesis in vitro tissue culture systems. Conclusion: Heparin mediated physically loaded VEGF onto the scaffolding biomaterials can release and promote angiogenesis in vitro tissue culture systems. It can be concluded that, VEGF containing HUVECs can stimulate directional micro-vessel network formation within a 3-D environment, such as a fibrin gel. References:[1] Harper, S.J., Bates, D.O., The key to anti-angiogenic therapeutics? Nature Reviews Cancer 8, 880-887 (2008).[2] Cabanas Danes, J., Huskens, J., Jonkheijm, P. Chemical strategies for the presentation and delivery of growth factors. J. Mater. Chem. B, 2014, 2, 2381 (2014).[3] Hadjizadeh, A., Doillon, C.J., Vermette, P., Bioactive polymer fibers to direct endothelial cell growth in a three-dimensional environment - Biomacromolecules, Biomacromolecules 8 (3), 864-873 (2007). Keywords: Tissue Engineering, growth factor, Surface modification, 3D scaffold Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: General Session Oral Topic: Regenerative medicine: biomaterials for control of tissue induction Citation: Hadjizadeh A and Savoji H (2016). Growth factor releasing micro strands promoted in vitro angiogenesis. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02875 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Afra Hadjizadeh Houman Savoji Google Afra Hadjizadeh Houman Savoji Google Scholar Afra Hadjizadeh Houman Savoji PubMed Afra Hadjizadeh Houman Savoji Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page." @default.
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• W2331862778 title "Growth factor releasing micro strands promoted in vitro angiogenesis" @default.
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